Detergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretion

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dc.contributorLab. Bioquímicapt_BR
dc.contributor.authorFuzita, Felipe J.pt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorPalmisano, Giuseppept_BR
dc.contributor.authorTerra, Walter R.pt_BR
dc.contributor.authorFerreira, Cléliapt_BR
dc.date.accessioned2020-07-09T21:24:04Z-
dc.date.available2020-07-09T21:24:04Z-
dc.date.issued2019pt_BR
dc.identifier.citationFuzita FJ., Pimenta DC, Palmisano G, Terra WR., Ferreira C. Detergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretion. Comp. Biochem. Physiol. B Biochem. Mol. Biol.. 2019 Sep;235:8-18. doi:10.1016/j.cbpb.2019.05.008.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2755-
dc.description.abstractThe midgut from lepidopteran insects has a particular way to release proteins to the lumen, named microapocrine secretion that could be an adaptation to release secretory contents into the lumen at water absorbing regions. In this process small vesicles (microapocrine vesicles) bud from the midgut microvilli as double membrane vesicles, where the inner membrane comes from the secretion vesicle and the outer one from the microvillar membrane. The molecular machinery associated with this process may be recruited by specific midgut microvilli membrane domains. To address to this, Spodoptera frugiperda midgut microvillar membranes, prepared by magnesium treatment and free from cytoskeleton with the hyperosmotic Tris procedure, were submitted to detergent extraction and fractionated by density gradient ultracentrifugation. Detergent-resistant membrane domains (DRM) were recovered and their proteins identified by proteomics. Microapocrine vesicles were isolated by washing the luminal surface of the midgut epithelium, followed by freezing and thawing plus centrifugation to recover only membranes. Proteins from purified microvillar membranes and microapocrine vesicle membranes were identified by proteomics. Comparison of the two populations suggests that the budding of microapocrine vesicles surrounded by microvillar membrane is not a random process, because only around 50% of the microvillar membrane proteins are in the microapocrine vesicles. From the 16 proteins from DRM, 14 were enriched in the microapocrine membrane vesicles. These results suggest that on budding, the microapocrine vesicle membrane is enclosed by DRM and a surrounding area of the microvillar membrane. It is proposed that the DRMs somehow recruit the proteins composing the secretory machinery.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent8-18pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofComparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biologypt_BR
dc.rightsRestricted accesspt_BR
dc.titleDetergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretionpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.cbpb.2019.05.008pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.cbpb.2019.05.008pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume235pt_BR
dc.subject.keywordMicrovillar proteomic analysispt_BR
dc.subject.keywordDetergent-resistant membrane domainspt_BR
dc.subject.keywordMicroapocrine secretionpt_BR
dc.relation.ispartofabbreviatedComp Biochem Physiol B Biochem Mol Biolpt_BR
dc.identifier.citationabntv. 235, p. 8-18, set. 2019pt_BR
dc.identifier.citationvancouver2019 Sep;235:8-18pt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bioquímica|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦441878/2014-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/08103-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/06863-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/14183-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.subject.researchlineToxinologia estruturalpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.grantfulltextembargo_29990101-
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