Behavior of alfa-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and Trypanosoma cruzi

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dc.contributor(LG) Laboratório de Genéticapt_BR
dc.contributor.authorEvangelista, Adriane Feijópt_BR
dc.contributor.authorKavati, Érica Akemipt_BR
dc.contributor.authorJankevicius, Jose Vitorpt_BR
dc.contributor.authorMenolli, Rafael Andradept_BR
dc.date.accessioned2020-07-09T21:24:09Z-
dc.date.available2020-07-09T21:24:09Z-
dc.date.issued2018pt_BR
dc.identifier.citationEvangelista AF, Kavati EA, Jankevicius JV, Menolli RA. Behavior of alfa-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and Trypanosoma cruzi. Acta Sci. Biol. Sci.. 2018;40:e41853. doi:10.4025/actascibiolsci.v40i1.41853.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2760-
dc.description.abstractGlycoalkaloids are important secondary metabolites accumulated by plants as protection against pathogens. One of them, a-tomatine, is found in high concentrations in green tomato fruits, while in the ripe fruits, its aglycone form, tomatidine, does not present a protective effect, and it is usual to find parasites of tomatoes like Phytomonasserpens in these ripe fruits. To investigate the sensitivity of trypanosomatids to the action of a-tomatine, we used logarithmic growth phase culture of 20 trypanosomatids from insects and plants and Trypanosoma cruzi. The lethal dose 50% (LD50) was determined by mixing 107 cells of the different isolates with a-tomatine at concentrations ranging from 10-3 to 10-8 M for 30 min at room temperature. The same tests performed with the tomatidine as a control showed no detectable toxicity against the same trypanosomatid cultures. The tests involved determination of the percentage (%) survival of the protozoan cultures in a Neubauer chamber using optical microscopy. The LD50 values varied from 10-4 to 10-6 M a-tomatine. Slight differences were detected among the LD50 values of the analyzed samples, and none of them showed evidence of resistance to the action of tomatinase, as shown by some pathogenic fungi.pt_BR
dc.format.extente41853pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofActa Scientiarum. Biological Sciencespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleBehavior of alfa-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and Trypanosoma cruzipt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.4025/actascibiolsci.v40i1.41853pt_BR
dc.identifier.urlhttps://doi.org/10.4025/actascibiolsci.v40i1.41853pt_BR
dc.contributor.externalHospital de Câncer de Barreto¦¦Brasilpt_BR
dc.contributor.external(UNIOESTE) Universidade Estadual do Oeste do Paranápt_BR
dc.identifier.citationvolume40pt_BR
dc.subject.keywordtomato alkaloidspt_BR
dc.subject.keywordtrypanocidal activitypt_BR
dc.subject.keywordPhytomonaspt_BR
dc.subject.keywordLeptomonaspt_BR
dc.subject.keywordCrithidiapt_BR
dc.subject.keywordHerpetomonaspt_BR
dc.relation.ispartofabbreviatedActa Sci Biol Scipt_BR
dc.identifier.citationabntv. 40, e41853, 2018pt_BR
dc.identifier.citationvancouver2018;40:e41853pt_BR
dc.contributor.butantanKavati, Érica Akemi|:Aluno|:Lab. Genética|:pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.grantfulltextopen-
item.openairetypeArticle-
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