Ultrastructural and cytotoxic effects of Metarhizium robertsii Infection on Rhipicephalus microplus hemocytes

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dc.contributorLab. Fisiopatologiapt_BR
dc.contributor.authorFiorotti, Jéssicapt_BR
dc.contributor.authorMenna-Barreto, Rubem Figueiredo Sadokpt_BR
dc.contributor.authorGolo, Patriciapt_BR
dc.contributor.authorCoutinho-Rodrigues, Caio Junior Balduinopt_BR
dc.contributor.authorBitencourt, Ricardo Oliveira Barbosapt_BR
dc.contributor.authorSpadacci-Morena, Diva Denellept_BR
dc.contributor.authorAngelo, Isabele da Costapt_BR
dc.contributor.authorBittencourt, Vânia Rita Elias Pinheiropt_BR
dc.date.accessioned2020-07-09T21:24:14Z-
dc.date.available2020-07-09T21:24:14Z-
dc.date.issued2019pt_BR
dc.identifier.citationFiorotti J, Menna-Barreto RFS, Golo P, Coutinho-Rodrigues CJB, Bitencourt ROB, Spadacci-Morena DD, et al. Ultrastructural and cytotoxic effects of Metarhizium robertsii Infection on Rhipicephalus microplus hemocytes. Front. physiol.. 2019 May;10:654. doi:10.3389/fphys.2019.00654.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2767-
dc.description.abstractMetarhizium is an entomopathogenic fungus widely employed in the biological control of arthropods. Hemocytes present in the hemolymph of invertebrates are the cells involved in the immune response of arthropods. Despite this, knowledge about Rhipicephalus microplus hemocytes morphological aspects as well as their role in response to the fungal infection is scarce. The present study aimed to analyze the hemocytes of R. microplus females after Metarhizium robertsii infection, using light and electron microscopy approaches associated with the cytotoxicity evaluation. Five types of hemocytes (prohemocytes, spherulocytes, plasmatocytes, granulocytes, and oenocytoids) were described in the hemolymph of uninfected ticks, while only prohemocytes, granulocytes, and plasmatocytes were observed in fungus-infected tick females. Twenty-four hours after the fungal infection, only granulocytes and plasmatocytes were detected in the transmission electron microscopy analysis. Hemocytes from fungus-infected tick females showed several cytoplasmic vacuoles with different electron densities, and lipid droplets in close contact to low electron density vacuoles, as well as the formation of autophagosomes and subcellular material in different stages of degradation could also be observed. M. robertsii propagules were more toxic to tick hemocytes in the highest concentration tested (1.0 × 108 conidia mL-1). Interestingly, the lowest fungus concentration did not affect significantly the cell viability. Microanalysis showed that cells granules from fungus-infected and uninfected ticks had similar composition. This study addressed the first report of fungal cytotoxicity analyzing ultrastructural effects on hemocytes of R. microplus infected with entomopathogenic fungi. These results open new perspectives for the comprehension of ticks physiology and pathology, allowing the identification of new targets for the biological control.pt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(FAPERJ) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiropt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent654pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Physiologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleUltrastructural and cytotoxic effects of Metarhizium robertsii Infection on Rhipicephalus microplus hemocytespt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fphys.2019.00654pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fphys.2019.00654pt_BR
dc.contributor.external(UFRRJ) Universidade Federal Rural do Rio de Janeiropt_BR
dc.contributor.external(FIOCRUZ) Fundação Oswaldo Cruzpt_BR
dc.identifier.citationvolume10pt_BR
dc.subject.keywordentomopathogenic fungipt_BR
dc.subject.keywordcell deathpt_BR
dc.subject.keywordfungal infectionpt_BR
dc.subject.keywordtickpt_BR
dc.subject.keywordimmunitypt_BR
dc.relation.ispartofabbreviatedFront physiolpt_BR
dc.identifier.citationabntv. 10, 654, maio 2019pt_BR
dc.identifier.citationvancouver2019 May;10:654pt_BR
dc.contributor.butantanSpadacci-Morena, Diva Denelle|:Pesquisador|:Lab. Fisiopatologia|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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