Proinflammatory effects of photoactivated methylene blue on rat model of Walker 256 carcinosarcoma

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dc.contributorLab. Biologia Molecularpt_BR
dc.contributor.authorPetrellis, M. Cpt_BR
dc.contributor.authorFrigo, L.pt_BR
dc.contributor.authorRibeiro, W.pt_BR
dc.contributor.authorLeal-Junior, E. C. P.pt_BR
dc.contributor.authorOliveira, F. R.pt_BR
dc.contributor.authorMaria, Durvanei Augustopt_BR
dc.contributor.authorLopes-Martins, R. Á. B.pt_BR
dc.identifier.citationPetrellis M.C, Frigo L., Ribeiro W., Leal-Junior E.C.P., Oliveira F.R., Maria DA, et al. Proinflammatory effects of photoactivated methylene blue on rat model of Walker 256 carcinosarcoma. Exp Oncol. 2019 Jun;41(2):112-122. doi:10.32471/exp-oncology.2312-8852.vol-41-no-2.13047.pt_BR
dc.description.abstractPhotodynamic therapy (PDT) is an anticancer therapy that associates the photosensitizer (PS), oxygen and light to destroy cancer cells. Methylene blue (MB) is considered a second generation phenothiazine dye with excellent photochemical properties. Aim: To evaluate whether MB-mediated PDT can induce oxidative stress and inflammation, therefore, interfering tumor growth. Materials and Methods: The study was conducted on Wistar rats transplanted with Walker 256 carcinosarcoma (W256). The proinflammatory interleukins levels (IL-1ß, IL-6, IL-10, TNF-a) were determined by ELISA, mRNA expression of COX-1, COX-2, iNOS and eNOS by RT-PCR, lipid peroxidation was measured by the TBARS method. Moreover, myeloperoxidase (MPO) activity in neutrophils was determined by MPO activity assay. All indices mentioned above were determined in tumor tissue. Kaplan — Meier and Gehan — Breslow — Wilcoxon tests were used for survival analysis. Results: We found that the treatment of W256 with 0.1% MB + 1 J/cm2 provoked a significant increase in the interleukins levels (IL-1ß, IL-6, IL-10, TNF-a), prostaglandin E2, the mRNA expression of COX-2, iNOS, lipid peroxidation and MPO activity in tumor tissue, which were statistically different (p < 0.05) compared to other experimental and control groups. The results of the estimation of survival curves show a greater probability of survival in 0.1% MB + 1 J/cm2 (total energy dose =142.8 J/cm2 ) treated group. Conclusion: Our results suggest that treatment of W256 with 0.1% MB + 1 J/cm2 was able to promote cytotoxic effects in tumor tissue by the generation of reactive oxygen species causing inflammation and thus interfering in the tumor growthpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extentp. 112-122pt_BR
dc.relation.ispartofExperimental Oncologypt_BR
dc.titleProinflammatory effects of photoactivated methylene blue on rat model of Walker 256 carcinosarcomapt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Guarulhos (UNG)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Nove de Julho (UNINOVE)¦¦Brasilpt_BR
dc.contributor.externalUniversidade do Vale do Paraíba (UNIVAP)¦¦Brasilpt_BR
dc.subject.keywordphotodynamic therapypt_BR
dc.subject.keywordWalker carcinosarcoma 256 cellspt_BR
dc.subject.keywordmethylene bluept_BR
dc.subject.keywordphotosensitizing agentpt_BR
dc.relation.ispartofabbreviatedExp Oncolpt_BR
dc.identifier.citationabntv. 41, n. 2, p. 112-122, jun. 2019pt_BR
dc.identifier.citationvancouver2019 Jun;41(2):112-122pt_BR
dc.contributor.butantanMaria, Durvanei Augusto|:Pesquisador|:Lab. Biologia Molecular|:pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦07/59124-0pt_BR
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