Comparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii

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dc.contributorLaboratório Especial de Ciclo Celularpt_BR
dc.contributor.authorSilva, Roberta Peres dapt_BR
dc.contributor.authorLongo, Larissa G. V.pt_BR
dc.contributor.authorda Cunha, Julia Pinheiro Chagaspt_BR
dc.contributor.authorSobreira, Tiago J. P.pt_BR
dc.contributor.authorRodrigues, Marcio L.pt_BR
dc.contributor.authorFaoro, Helissonpt_BR
dc.contributor.authorGoldenberg, Samuelpt_BR
dc.contributor.authorAlves, Lysangela R.pt_BR
dc.contributor.authorPuccia, Rosanapt_BR
dc.date.accessioned2020-07-09T21:24:54Z-
dc.date.available2020-07-09T21:24:54Z-
dc.date.issued2019-
dc.identifier.citationSilva RP, Longo LG.V., da Cunha JPC, Sobreira TJ.P., Rodrigues ML., Faoro H, et al. Comparison of the RNA Content of Extracellular Vesicles Derived from Paracoccidioides brasiliensis and Paracoccidioides lutzii. Cells. 2019 Jul;8(7):765. doi:10.3390/cells8070765.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2819-
dc.description.abstractParacoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from P. brasiliensis Pb18 and other pathogenic fungi. We have presently used the RNA-seq strategy to compare the <200- and >200-nt RNA fractions contained in EVs isolated from culture supernatants of P. brasiliensis Pb18, Pb3, and P. lutzii Pb01. Shared mRNA sequences were related toprotein modification, translation, and DNA metabolism/biogenesis, while those related to transport and oxidation-reduction were exclusive to Pb01. The presence of functional full-length mRNAs was validated by in vitro translation. Among small non-coding (nc)RNA, 15 were common to all samples; small nucleolar (sno)RNAs were enriched in P. brasiliensis EVs, whereas for P. lutzii there were similar proportions of snoRNA, rRNA, and tRNA. Putative exonic sRNAs were highly abundant in Pb18 EVs. We also found sRNA sequences bearing incomplete microRNA structures mapping to exons. RNA-seq data suggest that extracellular fractions containing Pb18 EVs can modulate the transcriptome of murine monocyte-derived dendritic cells in a transwell system. Considering that sRNA classes are involved in transcription/translation modulation, our general results may indicate that differences in virulence among fungal isolates can be related to their distinct EV-RNA contentpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação Oswaldo Cruzpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent765pt_BR
dc.languageengpt_BR
dc.relation.ispartofCellspt_BR
dc.rightsOpen Accesspt_BR
dc.titleComparison of the RNA content of extracellular vesicles derived from Paracoccidioides brasiliensis and Paracoccidioides lutziipt_BR
dc.typeArticlept_BR
dc.identifier.doihttps://repositorio.butantan.gov.br/handle/butantan/2819pt_BR
dc.identifier.urlhttps://doi.org/10.3390/cells8070765pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalPurdue University¦¦Estados Unidospt_BR
dc.contributor.externalInstituto Carlos Chagas (ICC). Fundação Oswaldo Cruz (FioCruz)¦¦Brasilpt_BR
dc.identifier.citationvolume8pt_BR
dc.identifier.citationissue7pt_BR
dc.subject.keywordParacoccidiodespt_BR
dc.subject.keywordextracellular vesiclespt_BR
dc.subject.keywordRNA-seqpt_BR
dc.subject.keywordmRNApt_BR
dc.subject.keywordsRNApt_BR
dc.relation.ispartofabbreviatedCellspt_BR
dc.identifier.citationabntv. 8, n. 7, p. 765, jul. 2019pt_BR
dc.identifier.citationvancouver2019 Jul;8(7):765pt_BR
dc.contributor.butantanda Cunha, Julia Pinheiro Chagas|:Pesquisador|:Laboratório Especial de Ciclo Celular|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦405520/2018-2pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦440015/2018-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301304/2017-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦10/19410-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦13/25950-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦10/19410-9pt_BR
dc.sponsorship.butantanFundação Oswaldo Cruz¦¦VPPCB-007-FIO-18-2-57pt_BR
dc.sponsorship.butantanFundação Oswaldo Cruz¦¦VPPIS-001-FIO-18-66pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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