Development and validation of shiga toxin-producing escherichia coli immunodiagnostic assay

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dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributor.authorSilva, Míriam Aparecida dapt_BR
dc.contributor.authorSantos, Anna Raquel Ribeiro dospt_BR
dc.contributor.authorRocha, Letícia Barbozapt_BR
dc.contributor.authorCaetano, Bruna Alvespt_BR
dc.contributor.authorMitsunari, Thaíspt_BR
dc.contributor.authorSantos, Luanda Iadocicco dospt_BR
dc.contributor.authorPolatto, Juliana M.pt_BR
dc.contributor.authorHorton, Denise Silvina Piccini Quintaspt_BR
dc.contributor.authorGuth, Beatriz E. C.pt_BR
dc.contributor.authorSantos, Luis Fernando dospt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.date.accessioned2020-07-09T21:24:56Z-
dc.date.available2020-07-09T21:24:56Z-
dc.date.issued2019-
dc.identifier.citationSilva MA, Santos ARR, Rocha LB, Caetano BA, Mitsunari T, Santos LI, et al. Development and validation of shiga toxin-producing escherichia coli immunodiagnostic assay. Microorganisms. 2019 Aug;7(9):276. doi:10.3390/microorganisms7090276.pt_BR
dc.identifier.issn2076-2607-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2822-
dc.description.abstractShiga toxin (Stx)–producing Escherichia coli (STEC) and its subgroup enterohemorrhagic E. coli are important pathogens involved in diarrhea, which may be complicated by hemorrhagic colitis and hemolytic uremic syndrome, the leading cause of acute renal failure in children. Early diagnosis is essential for clinical management, as an antibiotic treatment in STEC infections is not recommended. Previously obtained antibodies against Stx1 and Stx2 toxins were employed to evaluate the sensitivity and specificity of the latex Agglutination test (LAT), lateral flow assay (LFA), and capture ELISA (cEIA) for STEC detection. The LAT (mAb Stx1 plus mAb stx2) showed 99% sensitivity and 97% specificity. Individually, Stx1 antibodies showed 95.5% and 94% sensitivity and a specificity of 97% and 99% in the cEIA and LFA assay, respectively. Stx2 antibodies showed a sensitivity of 92% in both assays and a specificity of 100% and 98% in the cEIA and LFA assay, respectively. These results allow us to conclude that we have robust tools for the diagnosis of STEC infections.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent276pt_BR
dc.languageengpt_BR
dc.publisherMDPI AGpt_BR
dc.relation.ispartofMicroorganismspt_BR
dc.rightsOpen Accesspt_BR
dc.titleDevelopment and validation of shiga toxin-producing escherichia coli immunodiagnostic assaypt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/microorganisms7090276pt_BR
dc.identifier.urlhttps://doi.org/10.3390/microorganisms7090276pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.contributor.externalInstituto Adolfo Lutz (IAL)¦¦Brasilpt_BR
dc.publisher.cityBaselpt_BR
dc.identifier.citationvolume7pt_BR
dc.identifier.citationissue9pt_BR
dc.subject.keywordSTECpt_BR
dc.subject.keywordShiga toxinspt_BR
dc.subject.keywordantibodiespt_BR
dc.subject.keywordcapture ELISApt_BR
dc.subject.keywordlatex agglutination testpt_BR
dc.subject.keywordlateral flow assaypt_BR
dc.relation.ispartofabbreviatedMicroorganismspt_BR
dc.identifier.citationabntv. 7, n. 9, p. 276, aug. 2019pt_BR
dc.identifier.citationvancouver2019 Aug;7(9):276pt_BR
dc.publisher.countrySwitzerlandpt_BR
dc.contributor.butantanSilva, Míriam Aparecida da|:Técnico|:Laboratório de Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Bacteriologia|:Autor de correspondênciapt_BR
dc.contributor.butantanSantos, Anna Raquel Ribeiro dos|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanRocha, Letícia Barboza|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanCaetano, Bruna Alves|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanMitsunari, Thaís|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanSantos, Luanda Iadocicco dos|:|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanPolatto, Juliana M.|:Técnico|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanHorton, Denise Silvina Piccini Quintas|:Pesquisador|:Laboratório de Bacteriologia|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/06589-6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/50955-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/02618-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/090906-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.subject.researchlineToxinas microbianaspt_BR
item.openairetypeArticle-
item.grantfulltextembargo_29990101-
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