Molecular and morphological characterization of Hepatozoon spp. in Brazilian snakes

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dc.contributorMuseu Biológicopt_BR
dc.contributor.authorCalil, Priscila Rodriguespt_BR
dc.contributor.authorPuorto, Giuseppept_BR
dc.contributor.authorDunn, Jenny C.pt_BR
dc.contributor.authorChagas, Carolina Romeiro Fernandespt_BR
dc.contributor.authorRamos, Patrícia Locosquept_BR
dc.date.accessioned2020-07-09T21:25:15Z-
dc.date.available2020-07-09T21:25:15Z-
dc.date.issued2019pt_BR
dc.identifier.citationCalil PR, Puorto G, Dunn JC., Chagas CRF, Ramos PL. Molecular and morphological characterization of Hepatozoon spp. in Brazilian snakes. Amphib. reptil. 2019 Aug;40(3). doi:10.1163/15685381-20191113.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2843-
dc.description.abstractThe genus Hepatozoon represents one of six genera in the hemogregarine group. Some studies in snakes indicated effects in the host, from slight influences on fitness to severe effects on growth rate, reproduction and offspring survival rates. Diagnosis and identification are usually through blood smear analyses; but not all infected animals show parasitemia. Based on this, the present study established an adapted molecular protocol to identify Hepatozoon spp. to be used as a complementary test for routine diagnoses at the Clinical Analysis Laboratory at São Paulo Zoological Park Foundation. The study was conducted with 113 individuals. Microscopical analysis and molecular techniques were used to identify the parasite. Microscopic analyses showed 13.3% of the samples to be positive. The first pair of primers, targeting 18S rRNA gene, amplified parasite DNA in 6.3% of the samples. The second pair of primers, targeting Apicoplast fragment, were used only on samples that were identified microscopically as being positive, detecting the presence of parasite DNA in 93.3% of these. Phylogenetic analysis of the resulting sequences found five clusters for the 18S gene and five clusters for the Apicoplast fragment. Studies involving Hepatozoon spp. are still scarce and limited, mainly in snakes and the impacts of this parasite on the vertebrate host, so diagnostic studies are essential for wildlife conservation, especially in ex situ work.pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofAmphibia-reptilia : publication of the Societas Europaea Herpetologica.pt_BR
dc.titleMolecular and morphological characterization of Hepatozoon spp. in Brazilian snakespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1163/15685381-20191113pt_BR
dc.identifier.urlhttps://doi.org/10.1163/15685381-20191113pt_BR
dc.contributor.externalUniversidade Federal de São Carlos (UFSCar)¦¦Brasilpt_BR
dc.contributor.externalJoseph Banks Laboratories¦¦Inglaterrapt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume40pt_BR
dc.identifier.citationissue3pt_BR
dc.subject.keywordcaptivept_BR
dc.subject.keywordHepatozoonpt_BR
dc.subject.keywordPCRpt_BR
dc.subject.keywordremoparasitespt_BR
dc.subject.keywordreptilespt_BR
dc.subject.keywordsnakespt_BR
dc.relation.ispartofabbreviatedAmphib reptilpt_BR
dc.identifier.citationabntv. 40, n. 3, aug. 2019pt_BR
dc.identifier.citationvancouver2019 Aug;40(3)pt_BR
dc.contributor.butantanPuorto, Giuseppe|:Pesquisador|:Museu Biológico|:pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextSem Texto completo-
item.grantfulltextnone-
item.languageiso639-1English-
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