Proteomic analysis of soluble proteins retrieved from Duttaphrynus melanostictus skin secretion by IEx-batch sample preparation

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dc.contributorLab. Bioquímicapt_BR
dc.contributor.authorMariano, Douglas Oscar Ceolinpt_BR
dc.contributor.authorPrezotto-Neto, José Pedropt_BR
dc.contributor.authorSpencer, Patrick J.pt_BR
dc.contributor.authorSciani, Juliana Mozerpt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.identifier.citationMariano DOC, Prezotto-Neto JP, Spencer PJ., Sciani JM, Pimenta DC. Proteomic analysis of soluble proteins retrieved from Duttaphrynus melanostictus skin secretion by IEx-batch sample preparation. J. proteomics. 2019 Oct;209:103525. doi:10.1016/j.jprot.2019.103525.pt_BR
dc.description.abstractAmphibians display a toxic secretion that works as chemical defenses against predators and/or microorganismsthat is stored in specialized glands located in the tegument. For some animals, such glands have accumulated inspecific regions of the body and formed prominent structures known as macroglands. The Bufonidae familydisplays conspicuous macroglands in a post-orbital position, termed parotoids, which secretions are known to beextremely viscous and rich in alkaloids and steroids. Few proteins have been described in this material, though.Mainly, because of the difficulties to handle such biological matrix. In this context, we have performed a pro-teomic study on the parotoid macrogland secretion of the Asian bufonidDuttaphrynus melanostictus. By em-ploying the Ion-Exchange (IEx)-batch chromatography (anionic, cationic and both) we obtained six fractions -bound and unbound–that were submitted to an in solution-trypsin digestion followed by LC-MS/MS. Proteinsrelated to: antioxidant activity, binding processes (carbohydrate/lipid/protein), energy metabolism, hydrolases,lipid metabolism and membrane traffic were identified. Moreover, IEx was able to preserve the biological ac-tivity of the retrieved proteins (peptidasic). The current study increases the knowledge on the proteins present inthe bufonids parotoid macrogland secretion, providing a better understanding of the physiological role played bysuch molecules.Significance:The current approach allowed a detailed proteomic analysis of the several proteins synthesized intheD. melanostictusparotoid macrogland (Bufonidae) that are secreted into the skins, but embedded within acomplex viscous biological matrix. Moreover, our results aim to increase the knowledge on the biological roleplayed by such proteins at the skinpt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.relation.ispartofJournal of Proteomicspt_BR
dc.rightsRestricted accesspt_BR
dc.titleProteomic analysis of soluble proteins retrieved from Duttaphrynus melanostictus skin secretion by IEx-batch sample preparationpt_BR
dc.contributor.external(IPEN) Instituto de Pesquisas Energéticas e Nuclearespt_BR
dc.contributor.external(USF) Universidade São Franciscopt_BR
dc.subject.keywordamphibian skin secretionpt_BR
dc.subject.keywordDuttaphrynus melanostictuspt_BR
dc.subject.keywordIon-exchange chromatographypt_BR
dc.subject.keywordSample processingpt_BR
dc.relation.ispartofabbreviatedJ proteomicspt_BR
dc.identifier.citationabntv. 209, 103525, oct. 2019pt_BR
dc.identifier.citationvancouver2019 Oct;209:103525pt_BR
dc.contributor.butantanMariano, Douglas Oscar Ceolin|:Aluno|:Lab. Bioquímica|:PrimeiroAutorpt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bioquímica|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦406385/2018-1pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦303792/2016-7pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦969130pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/50040-4pt_BR
dc.subject.researchlineToxinologia estruturalpt_BR
item.fulltextCom Texto completo-
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