Cyclophosphamide treatment mimics sub-lethal infections with encephalitozoon intestinalis in immunocompromised individuals

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dc.contributorLab. Fisiopatologiapt_BR
dc.contributor.authorMoura, Maria Lucia Costa dept_BR
dc.contributor.authorAlvares-Saraiva, Anuska Marcelinopt_BR
dc.contributor.authorPerez, Elizabeth Cristinapt_BR
dc.contributor.authorXavier, José Guilhermept_BR
dc.contributor.authorSpadacci-Morena, Diva Denellept_BR
dc.contributor.authorMoysés, Carla Renata Serantonipt_BR
dc.contributor.authorRocha, Paulo Ricardo Dell’Armelinapt_BR
dc.contributor.authorLallo, Maria Anetept_BR
dc.date.accessioned2020-07-09T21:25:26Z-
dc.date.available2020-07-09T21:25:26Z-
dc.date.issued2019pt_BR
dc.identifier.citationMoura MLC, Alvares-Saraiva AM, Perez EC, Xavier JG, Spadacci-Morena DD, Moysés CRS, et al. Cyclophosphamide treatment mimics sub-lethal infections with encephalitozoon intestinalis in immunocompromised individuals. Front. microbiol.. 2019 Sep;10:2205. doi:10.3389/fmicb.2019.02205.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2857-
dc.description.abstractMicrosporidia, including Encephalitozoon intestinalis, are emerging pathogens which cause opportunistic infections in immunocompromised patients, such as those with AIDS, cancer, the elderly and people on immunosuppressive drugs. Intestinal mucosa (IM) is crucial for developing an efficient adaptive immune response against pathogenic micro-organisms, thereby preventing their colonization and subsequent infection. As immunosuppressive drugs affect the intestinal immune response is little known. In the present study, we investigated the immune response to E. intestinalis infection in the IM and gut-associated lymphoid tissue (GALT) in cyclophosphamide (Cy) immunosuppressed mice, to mimic an immunocompromised condition. Histopathology revealed lymphoplasmacytic enteritis at 7 and 14 days-post-infection (dpi) in all infected groups, however, inflammation diminished at 21 and 28 dpi. Cy treatment also led to a higher number of E. intestinalis spores and lesions, which reduced at 28 dpi. In addition, flow cytometry analysis demonstrated CD4+ and CD8+ T cells to be predominant immune cells, with up-regulation in both Th1 and Th2 cytokines at 7 and 14 dpi, as demonstrated by histopathology. In conclusion, Cy treatment reduced GALT (Peyer’s plaques and mesenteric lymph nodes) and peritoneum populations but increased the T-cell population in the intestinal mucosa and the production of pro-and anti-inflammatory cytokines, which were able to eliminate this opportunistic fungus and reduced the E. intestinalis infection.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent2205pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleCyclophosphamide treatment mimics sub-lethal infections with encephalitozoon intestinalis in immunocompromised individualspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3389/fmicb.2019.02205pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fmicb.2019.02205pt_BR
dc.contributor.externalUniversidade Paulista (UNIP)¦¦Brasilpt_BR
dc.identifier.citationvolume10pt_BR
dc.subject.keywordEncephalitozoon intestinalispt_BR
dc.subject.keywordmicrosporidiapt_BR
dc.subject.keywordmucosal immunitypt_BR
dc.subject.keywordintestinal inflammationpt_BR
dc.subject.keywordcyclophosphamidept_BR
dc.relation.ispartofabbreviatedFront microbiolpt_BR
dc.identifier.citationabntv. 10, 2205, sep. 2019pt_BR
dc.identifier.citationvancouver2019 Sep;10:2205pt_BR
dc.contributor.butantanSpadacci-Morena, Diva Denelle|:Pesquisador|:Lab. Fisiopatologia|:pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/25948-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/51727-5pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.languageiso639-1English-
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