Crotalus durissus ruruima snake venom and a Phospholipase A2 isolated from this venom elicit macrophages to form lipid droplets and synthesize inflammatory lipid mediators

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dc.contributorLab. Farmacologiapt_BR
dc.contributor.authorCarvalho, Ana Eduarda Zulim dept_BR
dc.contributor.authorGiannotti, Karina Cristinapt_BR
dc.contributor.authorLeiguez, Elbiopt_BR
dc.contributor.authorMatsubara, Márcio Hidekipt_BR
dc.contributor.authorSantos, Maria Cristina Dospt_BR
dc.contributor.authorFortes-Dias, Consuelo Latorrept_BR
dc.contributor.authorTeixeira, Catarina de Fátima Pereirapt_BR
dc.date.accessioned2020-07-09T21:25:49Z-
dc.date.available2020-07-09T21:25:49Z-
dc.date.issued2019pt_BR
dc.identifier.citationCarvalho AEZ, Giannotti KC, Leiguez E, Matsubara MH, Santos MCD, Fortes-Dias CL, et al. Crotalus durissus ruruima snake venom and a Phospholipase A2 isolated from this venom elicit macrophages to form lipid droplets and synthesize inflammatory lipid mediators. J. Immunol. Res.. 2019 Nov;2019:2745286. doi:10.1155/2019/2745286.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2885-
dc.description.abstractViper snake Crotalus durissus ruruima (Cdr) is a subspecies found in northern area of Brazil. Among the snakes of Crotalus genus subspecies, the venom of Cdr presents highest level of crotoxin, which is the major component of Crotalus snake venoms, formed by two subunits (crotapotin and a phospholipase A2 named CBr) and presents potent neurotoxic activity. Curiously, the venom of C. d. ruruima (CdrV) is better neutralized by antibothropic than by anticrotalic serum, strongly suggesting that this venom has similarities with venom of Bothrops genus snakes with regard to the ability to induce inflammation. Macrophages are cells with a central role in inflammatory and immunological responses. Upon inflammatory stimuli, these cells exhibit increased numbers of lipid droplets, which are key organelles in the synthesis and release of inflammatory mediators. However, the effects of CdrV and CBr in macrophage functions are unknown. We herein investigated the ability of CdrV and CBr to activate macrophages with focus on the formation of lipid droplets (LDs), synthesis of lipid mediators, and mechanisms involved in these effects. The involvement of LDs in PGE2 biosynthesis was also assessed. Stimulation of murine macrophages with CdrV and CBr induced an increased number of LDs and release of prostanoids (PGE2, PGD2, and TXB2). Neither CdrV nor CBr induced the expression of COX-1 and COX-2 by macrophages. LDs induced by both CdrV and CBr are associated to PLIN2 recruitment and expression and were shown to be dependent on COX-1, but not COX-2 activity. Moreover, PGE2 colocalized to CdrV- and CBr-induced LDs, revealing the role of these organelles as sites for the synthesis of prostanoids. These results evidence, for the first time, the ability of a whole snake venom to induce formation of LDs and the potential role of these organelles for the production of inflammatory mediators during envenomation by Crotalus snakes.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FAPEMIG) Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.format.extent2745286pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Immunology Researchpt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleCrotalus durissus ruruima snake venom and a Phospholipase A2 isolated from this venom elicit macrophages to form lipid droplets and synthesize inflammatory lipid mediatorspt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1155/2019/2745286pt_BR
dc.identifier.urlhttps://doi.org/10.1155/2019/2745286pt_BR
dc.contributor.external(UEA) Universidade do Estado do Amazonaspt_BR
dc.contributor.external(FUNED) Fundação Ezequiel Diaspt_BR
dc.identifier.citationvolume2019pt_BR
dc.relation.ispartofabbreviatedJ Immunol Respt_BR
dc.identifier.citationabntv. 2019, p. 2745286, nov. 2019pt_BR
dc.identifier.citationvancouver2019 Nov;2019:2745286pt_BR
dc.contributor.butantanCarvalho, Ana Eduarda Zulim de|:Aluno|:|:PrimeiroAutorpt_BR
dc.contributor.butantanTeixeira, Catarina de Fátima Pereira|:Pesquisador:Docente Permanente PPGTOX|:Lab. Farmacologia|:Autor de correspondênciapt_BR
dc.contributor.butantanGiannotti, Karina Cristina|:Aluno|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanLeiguez, Elbio|:Aluno|:Lab. Farmacologia|:pt_BR
dc.contributor.butantanMatsubara, Márcio Hideki|:Aluno|:|:pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦307379/2016-7pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦1667206pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦BIP-00182-18pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/18549-1pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/50040-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦18/05637-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/24701-3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦00/11624-5pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.subject.researchlineBioprospecção e desenvolvimentopt_BR
dc.description.dbindexedYespt_BR
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item.languageiso639-1English-
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