Culture-attenuated pathogenic Leptospira lose the ability to survive to complement-mediated-killing due to lower expression of factor H binding proteins

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dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributor.authorMoreno-Torres, Azafpt_BR
dc.contributor.authorMalvido-Jiménez, Irving R.pt_BR
dc.contributor.authorPeña-Moctezuma, Alejandro de lapt_BR
dc.contributor.authorSánchez, Luz O. Castillopt_BR
dc.contributor.authorFraga, Tatiana R.pt_BR
dc.contributor.authorBarbosa, Angela Silvapt_BR
dc.contributor.authorIsaac, Lourdespt_BR
dc.contributor.authorSahagún-Ruiz, Alfredopt_BR
dc.date.accessioned2020-07-09T21:25:52Z-
dc.date.available2020-07-09T21:25:52Z-
dc.date.issued2019-
dc.identifier.citationMoreno-Torres A, Malvido-Jiménez IR., Peña-Moctezuma A, Sánchez LO.C, Fraga TR., Barbosa AS, et al. Culture-attenuated pathogenic Leptospira lose the ability to survive to complement-mediated-killing due to lower expression of factor H binding proteins. Microbes Infect.. 2019 Oct-Nov;21(8-9):377-385. doi:10.1016/j.micinf.2019.03.001.pt_BR
dc.identifier.issn1286-4579-
dc.identifier.issn1769-714X-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2889-
dc.description.abstractSeveral pathogens including Gram-negative bacteria hijack complement regulators to escape host's innate response. Pathogenic Leptospira species bind Factor H, C4b binding protein and vitronectin from the complement system. We evaluated the ability of low passage (LP) and culture-attenuated (CA) pathogenic strains of Leptospira, to bind Factor H. We used LOCaS46 (Leptospira interrogans sv Canicola), LOVe30 (L. interrogans sv Icterohaemorrhagiae) and MOCA45 (L. santarosai sv Tarassovi), and ten high passage strains of Leptospira [used in the microscopic agglutination test (MAT)]. Afterwards, we assessed their survival in normal human serum (NHS). Interestingly, the ability in binding Factor H was higher for LOCaS46 and LOVe30 LP strains, than for the respective CA strains suggesting that the ability of evading the alternative complement pathway is lost after culture attenuation. Accordingly, the level of mRNA expression of the Factor H binding proteins, LigA, LigB and Lsa23 was higher in these LP strains than in the corresponding CA strains. Unexpectedly, no difference in Factor H binding and surviving was observed between LP and CA MOCA45 strains. The high passage MAT-reference strains showed variation in Factor H binding ability, but, in most cases, the ability for capturing Factor H by Leptospira strains correlated with their survival in NHS.pt_BR
dc.description.sponsorshipConsejo Nacional de Ciencia y Tecnología (CONACYT)pt_BR
dc.description.sponsorshipUniversidad Nacional Autónoma de México (UNAM)pt_BR
dc.format.extent377-385pt_BR
dc.languageengpt_BR
dc.publisherElsevierpt_BR
dc.relation.ispartofMicrobes and Infectionpt_BR
dc.titleCulture-attenuated pathogenic Leptospira lose the ability to survive to complement-mediated-killing due to lower expression of factor H binding proteinspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.micinf.2019.03.001pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.micinf.2019.03.001pt_BR
dc.contributor.externalUniversidad Nacional Autónoma de México (UNAM)¦¦Méxicopt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.publisher.cityParispt_BR
dc.identifier.citationvolume21pt_BR
dc.identifier.citationissue8-9pt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordFactor Hpt_BR
dc.subject.keywordComplement evasionpt_BR
dc.subject.keywordLeptospira complement binding proteinspt_BR
dc.relation.ispartofabbreviatedMicrobes Infect.pt_BR
dc.identifier.citationabntv. 21, n. 8-9, p. 377-385, oct-nov. 2019pt_BR
dc.identifier.citationvancouver2019 Oct-Nov;21(8-9):377-385pt_BR
dc.publisher.countryFrancept_BR
dc.contributor.butantanBarbosa, Angela Silva|:Pesquisador|:Laboratório de Bacteriologia|:pt_BR
dc.sponsorship.butantanConsejo Nacional de Ciencia y Tecnología (CONACYT)¦¦pt_BR
dc.sponsorship.butantanUniversidad Nacional Autónoma de México (UNAM)¦¦PAPIIT-IN222214pt_BR
dc.sponsorship.butantanUniversidad Nacional Autónoma de México (UNAM)¦¦PAPIIT-IN224717pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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item.grantfulltextembargo_29990101-
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