Evaluation of inactivated Bordetella pertussis as a delivery system for the immunization of mice with Pneumococcal Surface Antigen A

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dc.contributorLab. Bacteriologiapt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorCastro, Júlia Tavares dept_BR
dc.contributor.authorOliveira, Giuliana Stephani dept_BR
dc.contributor.authorNishigasako, Melissa Akemipt_BR
dc.contributor.authorDebrie, Anne-Sophiept_BR
dc.contributor.authorMiyaji, Eliane Namiept_BR
dc.contributor.authorSchanoski, Alessandra Soarespt_BR
dc.contributor.authorAkamatsu, Milena Apetitopt_BR
dc.contributor.authorLocht, Camillept_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorMielcarek, Nathaliept_BR
dc.contributor.authorOliveira, Maria Leonor Sarno dept_BR
dc.date.accessioned2020-07-09T21:26:05Z-
dc.date.available2020-07-09T21:26:05Z-
dc.date.issued2020pt_BR
dc.identifier.citationCastro JT, Oliveira GS, Nishigasako MA, Debrie A-S, Miyaji EN, Schanoski AS, et al. Evaluation of inactivated Bordetella pertussis as a delivery system for the immunization of mice with Pneumococcal Surface Antigen A. PloS One. 2020 Jan;15(2):e0229050. doi:10.1371/journal.pone.0228055.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2908-
dc.description.abstractPneumococcal Surface Protein A (PspA) has been successfully tested as vaccine candidate against Streptococcus pneumoniae infections. Vaccines able to induce PspA-specific antibodies and Th1 cytokines usually provide protection in mice. We have shown that the whole cell pertussis vaccine (wP) or components from acellular pertussis vaccines, such as Pertussis Toxin or Filamentous Hemagglutinin (FHA), are good adjuvants to PspA, suggesting that combined pertussis-PspA vaccines would be interesting strategies against the two infections. Here, we evaluated the potential of wP as a delivery vector to PspA. Bordetella pertussis strains producing a PspA from clade 4 (PspA4Pro) fused to the N-terminal region of FHA (Fha44) were constructed and inactivated with formaldehyde for the production of wPPspA4Pro. Subcutaneous immunization of mice with wPPspA4Pro induced low levels of anti-PspA4 IgG, even after 3 doses, and did not protect against a lethal pneumococcal challenge. Prime-boost strategies using wPPspA4Pro and PspA4Pro showed that there was no advantage in using the wPPspA4Pro vaccine. Immunization of mice with purified PspA4Pro induced higher levels of antibodies and protection against pneumococcal infection than the prime-boost strategies. Finally, purified Fha44:PspA4Pro induced high levels of anti-PspA4Pro IgG, but no protection, suggesting that the antibodies induced by the fusion protein were not directed to protective epitopes.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extente0228055pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleEvaluation of inactivated Bordetella pertussis as a delivery system for the immunization of mice with Pneumococcal Surface Antigen Apt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.1371/journal.pone.0228055pt_BR
dc.identifier.urlhttps://doi.org/10.1371/journal.pone.0228055pt_BR
dc.contributor.externalInstitut Pasteur de Lille¦¦Françapt_BR
dc.identifier.citationvolume15pt_BR
dc.identifier.citationissue1pt_BR
dc.relation.ispartofabbreviatedPloS Onept_BR
dc.identifier.citationabntv. 15, n. 2, e0229050, jan. 2020pt_BR
dc.identifier.citationvancouver2020 Jan;15(2):e0229050pt_BR
dc.contributor.butantanCastro, Júlia Tavares de|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanOliveira, Giuliana Stephani de|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanNishigasako, Melissa Akemi|:Aluno|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanMiyaji, Eliane Namie|:Pesquisador|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanSchanoski, Alessandra Soares|:Pesquisador|:Lab. Bacteriologia|:pt_BR
dc.contributor.butantanAkamatsu, Milena Apetito|:Pesquisador|:Centro Bioindustrialpt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrial|:pt_BR
dc.contributor.butantanOliveira, Maria Leonor Sarno de|:Pesquisador|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦301856/2016-8pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/13134-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/17258-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01992-8pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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