Tetanus antitoxin potency assessment by surface plasmon resonance and ToBI test

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dc.contributorLab. Biofármacospt_BR
dc.contributorLab. Virologiapt_BR
dc.contributorSeção de Processamento de Plasmas Hiperimunespt_BR
dc.contributor.authorQuintilio, Wagnerpt_BR
dc.contributor.authorKapronezai, Josanapt_BR
dc.contributor.authorTakata, Daniela Yumipt_BR
dc.contributor.authorMarcelino, José Robertopt_BR
dc.contributor.authorMoro, Ana Mariapt_BR
dc.date.accessioned2020-07-09T21:26:09Z-
dc.date.available2020-07-09T21:26:09Z-
dc.date.issued2019pt_BR
dc.identifier.citationQuintilio W, Kapronezai J, Takata DY, Marcelino JR, Moro AM. Tetanus antitoxin potency assessment by surface plasmon resonance and ToBI test. Biologicals. 2019 Nov;62:107-110. doi:10.1016/j.biologicals.2019.09.005.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2914-
dc.description.abstractPotency testing of tetanus antitoxin must be performed in vivo, in a very painful, stressful and prone to high variability assay. It is, therefore, mandatory to find alternatives to this kind of potency assessment. Immunochemical tests as ELISA or ToBI test are already available but usually results in a poor correlation to the in vivo protection. Considering research and development of mono and oligoclonal antibodies against tetanus and the improvement of equine polyclonal antitoxin production and control, we developed an alternative instrumental test for tetanus antitoxin by using surface plasmon resonance. Tetanus antitoxin from hyperimmune equine sera (16 batches) were tested and the results indicated excellent concordance and correlation to the in vivo test (Lin's Ró=0.9). This innovative approach should now be improved in order to extend it to oligoclonal and monoclonal human antibodies aiming to replace mice for the potency assessment of tetanus antitoxin especially during research and development steps.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent107-110pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBiologicalspt_BR
dc.titleTetanus antitoxin potency assessment by surface plasmon resonance and ToBI testpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.biologicals.2019.09.005pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.biologicals.2019.09.005pt_BR
dc.identifier.citationvolume62pt_BR
dc.subject.keywordSurface plasmon resonancept_BR
dc.subject.keywordTetanus antitoxinpt_BR
dc.subject.keywordToxin binding inhibition testpt_BR
dc.relation.ispartofabbreviatedBiologicalspt_BR
dc.identifier.citationabntv. 62, p. 107-110, nov. 2019pt_BR
dc.identifier.citationvancouver2019 Nov;62:107-110pt_BR
dc.contributor.butantanQuintilio, Wagner|:Pesquisador|:Lab. Biofármacos |:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanKapronezai, Josana|:Técnico|:Lab. Virologia|:pt_BR
dc.contributor.butantanTakata, Daniela Yumi|:Aluno|:Lab. Biofármacos |:pt_BR
dc.contributor.butantanMarcelino, José Roberto|:Técnico|:Seção de Processamento de Plasmas Hiperimunes|:pt_BR
dc.contributor.butantanMoro, Ana Maria|:Pesquisador|:Lab. Biofármacos |:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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crisitem.author.orcid0000-0002-3382-8184-
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crisitem.author.orcid0000-0002-0650-7764-
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