Inflammatory reaction induced by two metalloproteinases isolated from bothrops atrox venom and by fragments generated from the hydrolysis of basement membrane components

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dc.contributorLaboratório de Imunopatologiapt_BR
dc.contributorLaboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorAlmeida, Michelle Teixeira dept_BR
dc.contributor.authorFreitas-de-Sousa, Luciana Aparecidapt_BR
dc.contributor.authorColombini, Mônicapt_BR
dc.contributor.authorGimenes, Sarah Natalie Cirilopt_BR
dc.contributor.authorKitano, Eduardo Shigueopt_BR
dc.contributor.authorFaquim Mauro, Eliana Limapt_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.contributor.authorMoura-da-Silva, Ana Mariapt_BR
dc.date.accessioned2020-07-09T21:26:23Z-
dc.date.available2020-07-09T21:26:23Z-
dc.date.issued2020-
dc.identifier.citationAlmeida MT, Freitas-de-Sousa LA, Colombini M, Gimenes SNC, Kitano ES, Faquim Mauro EL, et al. Inflammatory reaction induced by two metalloproteinases isolated from bothrops atrox venom and by fragments generated from the hydrolysis of basement membrane components. Toxins. 2020 Feb;12(2):96. doi:10.3390/toxins12020096.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2930-
dc.description.abstractSnake venom metalloproteinases (SVMPs) play an important role in local tissue damage of snakebite patients, mostly by hydrolysis of basement membrane (BM) components. We evaluated the proinflammatory activity of SVMPs Atroxlysin-Ia (ATXL) and Batroxrhagin (BATXH) from Bothrops atrox venom and their hydrolysis products of Matrigel. BALB/c mice were injected with SVMPs (2 µg), for assessment of paw edema and peritoneal leukocyte accumulation. Both SVMPs induced edema, representing an increase of ~70% of the paw size. Leukocyte infiltrates reached levels of 6 × 106 with ATXL and 5 × 106 with BATXH. TNF-a was identified in the supernatant of BATXH—or venom-stimulated MPAC cells. Incubation of Matrigel with the SVMPs generated fragments, including peptides from Laminin, identified by LC–MS/MS. The Matrigel hydrolysis peptides caused edema that increased 30% the paw size and promoted leukocyte accumulation (4–5 × 106) to the peritoneal cavity, significantly higher than Matrigel control peptides 1 and 4 h after injection. Our findings suggest that ATXL and BATXH are involved in the inflammatory reaction observed in B. atrox envenomings by direct action on inflammatory cells or by releasing proinflammatory peptides from BM proteins that may amplify the direct action of SVMPs through activation of endogenous signaling pathwayspt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent96pt_BR
dc.languageengpt_BR
dc.relation.ispartofToxinspt_BR
dc.rightsOpen Accesspt_BR
dc.titleInflammatory reaction induced by two metalloproteinases isolated from bothrops atrox venom and by fragments generated from the hydrolysis of basement membrane componentspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/toxins12020096pt_BR
dc.identifier.urlhttps://doi.org/10.3390/toxins12020096pt_BR
dc.identifier.citationvolume12pt_BR
dc.identifier.citationissue2pt_BR
dc.subject.keywordBothrops atroxpt_BR
dc.subject.keywordSVMPspt_BR
dc.subject.keywordmetalloproteinasespt_BR
dc.subject.keywordbasal membranept_BR
dc.subject.keywordhydrolysispt_BR
dc.subject.keywordpeptidespt_BR
dc.subject.keywordinflammationpt_BR
dc.relation.ispartofabbreviatedToxinspt_BR
dc.identifier.citationabntv. 12, n. 2, p. 96, fev. 2020pt_BR
dc.identifier.citationvancouver2020 Feb;12(2):96pt_BR
dc.contributor.butantanAlmeida, Michelle Teixeira de|:Aluno|:Laboratório de Imunopatologia|:PrimeiroAutorpt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente Permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanKitano, Eduardo Shigueo|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:pt_BR
dc.contributor.butantanFreitas-de-Sousa, Luciana Aparecida|:Aluno|:Laboratório de Imunopatologia|:pt_BR
dc.contributor.butantanColombini, Mônica|:Técnico|:Laboratório de Imunopatologia|:pt_BR
dc.contributor.butantanGimenes, Sarah Natalie Cirilo|:Aluno|:Laboratório de Imunopatologia|:pt_BR
dc.contributor.butantanFaquim-Mauro, Eliana|:Pesquisador|:Laboratório de Imunopatologia|:pt_BR
dc.contributor.butantanMoura-da-Silva, Ana Maria|:Docente Permanente PPGTOX|:Laboratório de Imunopatologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦303958/2018-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦308133/2015-3pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦063/2010pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦1209/2011pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50127-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/26058-8pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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