Nuclear export of replication protein A in the non-replicative infective forms of Trypanosoma cruzi


Publication type
Article
Language
English
Appears in Collections:
Metrics
Abstract
Replication Protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA-binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a Nuclear Export Signal (NES) in the trypanosomatid RPA-2 subunit. Mutations in the negatively charged residues of RPA-2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms.
Reference
Pavani RS, Lima LPO, Lima AA, Fernandes CA.H., Fragoso SP., Calderano SG, et al. Nuclear export of replication protein A in the non-replicative infective forms of Trypanosoma cruzi. FEBS Lett.. 2020 Feb. doi:10.1002/1873-3468.13755.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/2945
Journal title
Issue Date
2020


Files in This Item:

Existing users please Login
10.10021873-3468.13755.pdf
Size: 2.96 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show full item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.