Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection

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dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributor.authorVieira, Paulo Cesar Gomespt_BR
dc.contributor.authorEspinoza-Culupú, Abraham Omarpt_BR
dc.contributor.authorNepomuceno, Robertopt_BR
dc.contributor.authorAlves, Marina Ramospt_BR
dc.contributor.authorLebrun, Ivopt_BR
dc.contributor.authorElias, Waldir Pereirapt_BR
dc.contributor.authorRuiz, Rita de Cássiapt_BR
dc.identifier.citationVieira PCG, Espinoza-Culupú AO, Nepomuceno R, Alves MR, Lebrun I, Elias WP, et al. Secreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infection. PloS One. 2020 Feb;15(2):e0228959. doi:10.1371/journal.pone.0228959.pt_BR
dc.description.abstractSecreted autotransporter toxin (Sat) is a 107-kDa serine protease autotransporter of Enterobacteriaceae (SPATE) presenting cytotoxic activity in renal and bladder cells. Further studies have detected the Sat-encoding gene (sat) in enteroaggregative Escherichia coli (EAEC) and in E. coli strains isolated from neonatal septicemia and meningitis. Here, we investigated the role of Sat as a cytotoxin of EAEC. Sat was purified from a strain of E. coli harboring sat (DEC/Sat+, O126:H2) and used to raise antibodies in rabbit. The presence of Sat was detected by ELISA in the supernatant of 93.7% of EAEC strains harboring sat and in none lacking the gene. The effect of Sat during infection was investigated in polarized Caco-2 cells infected with Sat-producing EAEC (CV323/77,O125ab:H21). This strain induced intense cell detachment, which was inhibited by PMSF or Sat antiserum. Also, sat transcription and Sat production were detected during infection. Here we demonstrate that Sat is internalized in polarized cells leading to F-actin disruption which preceded cell detachment. A comparative study of the toxin action in cell lines corresponding to the infection sites in which bacteria carrying the sat gene have been isolated was performed. Cells originating from the gastrointestinal tract (Caco-2), urinary (LLC-PK1) and endothelium (HUVEC) were incubated with purified Sat. The time required for observation of cell damage differed according to the cell line. HUVEC cells were more sensitive to Sat than cells derived from urinary and intestinal tracts. The intense activity of Sat on the endothelial cells suggests that Sat could also be a virulence factor for the bacteria in the bloodstream. In addition, this is the first work demonstrating that Sat induces cytotoxic effect during EAEC infection in vitro. The cell damage observed during infection indicates that Sat may be another toxin with cytotoxic role in the EAEC pathogenesis.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.relation.ispartofPloS Onept_BR
dc.rightsOpen Accesspt_BR
dc.titleSecreted autotransporter toxin (Sat) induces cell damage during enteroaggregative Escherichia coli infectionpt_BR
dc.relation.ispartofabbreviatedPloS Onept_BR
dc.identifier.citationabntv. 15, n. 2, e0228959, fev. 2020pt_BR
dc.identifier.citationvancouver2020 Feb;15(2):e0228959pt_BR
dc.contributor.butantanVieira, Paulo Cesar Gomes|:Técnico|:Laboratório de Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanLebrun, Ivo|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanEspinoza-Culupú, Abraham Omar|:|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanNepomuceno, Roberto|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanAlves, Marina Ramos|:Aluno|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanElias, Waldir Pereira|:Pesquisador|:Laboratório de Bacteriologia|:pt_BR
dc.contributor.butantanRuiz, Rita de Cássia|:Pesquisador|:Laboratório de Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/11212-9pt_BR
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