Leptospira interrogans Bat proteins impair host hemostasis by fibrinogen cleavage and platelet aggregation inhibition

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dc.contributorLaboratório de Desenvolvimento de Vacinaspt_BR
dc.contributorLaboratório de Dor e Sinalizaçãopt_BR
dc.contributor.authorPassalia, Felipe Josépt_BR
dc.contributor.authorHeinemann, Marcos Bryanpt_BR
dc.contributor.authorChudzinski, Sonia Aparecida de Andradept_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.contributor.authorVieira, Mônica Laruccipt_BR
dc.date.accessioned2020-07-09T21:26:41Z-
dc.date.available2020-07-09T21:26:41Z-
dc.date.issued2020pt_BR
dc.identifier.citationPassalia FJ, Heinemann MB, Chudzinski SAA, Nascimento ALTO, Vieira ML. Leptospira interrogans Bat proteins impair host hemostasis by fibrinogen cleavage and platelet aggregation inhibition. Med. Microbiol. Immunol.. 2020 Feb;209:201-213. doi:10.1007/s00430-020-00664-4.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2953-
dc.description.abstractLeptospirosis is a worldwide spread zoonosis, caused by pathogenic Leptospira. Evidences suggest that compromised hemostasis might be involved in the leptospirosis pathophysiology. In the genome of L. interrogans serovar Copenhageni, we found two genes coding for proteins which comprise von Willebrand factor (VWF) A domains (BatA and BatB). As VWF A domains exhibit multiple binding sites which contributes to human VWF hemostatic functions, we hypothesized that the L. interrogans BatA and BatB proteins could be involved in the hemostatic impairment during leptospirosis. We have cloned, expressed in Escherichia coli, and purified recombinant BatA and BatB. The influence of recombinant BatA and BatB on different in vitro hemostatic assays evaluating the enzymatic activity, platelet aggregation and fibrinogen integrity was investigated. We describe BatB as a new serine protease which is able to cleave thrombin chromogenic substrate, fibrin, fibrinogen, gelatin and casein; while BatA is active only towards fibrinogen. BatA and BatB interfere with the platelet aggregation induced by VWF/ristocetin and thrombin. Our results suggest an important role of the L. interrogans serovar Copenhageni Bat proteins in the hemostasis dysfunction observed during leptospirosis and contribute to the understanding of the leptospirosis pathophysiological mechanisms.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent201-213pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofMedical Microbiology and Immunologypt_BR
dc.titleLeptospira interrogans Bat proteins impair host hemostasis by fibrinogen cleavage and platelet aggregation inhibitionpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1007/s00430-020-00664-4pt_BR
dc.identifier.urlhttps://doi.org/10.1007/s00430-020-00664-4pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de Minas Gerais (UFMG)¦¦Brasilpt_BR
dc.identifier.citationvolume209pt_BR
dc.subject.keywordLeptospirosispt_BR
dc.subject.keywordvon Willebrand factorpt_BR
dc.subject.keywordSerine proteasespt_BR
dc.subject.keywordPlatelet aggregationpt_BR
dc.subject.keywordInfectious diseasespt_BR
dc.subject.keywordHemostasispt_BR
dc.relation.ispartofabbreviatedMed Microbiol Immunolpt_BR
dc.identifier.citationabntv. 209, p. 201-213, fev. 2020pt_BR
dc.identifier.citationvancouver2020 Feb;209:201-213pt_BR
dc.contributor.butantanPassalia, Felipe José|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:PrimeiroAutorpt_BR
dc.contributor.butantanChudzinski, Sonia Aparecida de Andrade|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório de Dor e Sinalização|:pt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório de Desenvolvimento de Vacinas|:pt_BR
dc.contributor.butantanVieira, Mônica Larucci|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/00236-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/50981-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/07054-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01102-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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