Development of an enzyme-linked immunosorbent assay for diagnosis of human herpesvirus-7 infection

INTRODUCTION: Human herpesvirus (HHV)-7 establishes a latent infection during the lifetime of the host and can reactivate after the primary infection, leading to lytic replication in immunosuppressed patients. METHODS :This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) to identify HHV-7 serum antibodies and compare its performance with that of an indirect immunofluorescence assay (IFA). RESULTS: Serum samples (n=102) were tested by IgG-IFA and by ELISA. IFA and ELISA showed IgG-positive results in 77 and 73 samples, respectively. Qualitative concordance of 96% was demonstrated between the two techniques. CONCLUSIONS: ELISA may be useful to diagnose HHV-7 infection.
Keywords
ELISA;  IFA;  HHV-7;  Serological Assay

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Puglia ALP, Peigo MF, Bomfim FRC, Thomasini RL. Development of an enzyme-linked immunosorbent assay for diagnosis of human herpesvirus-7 infection. Rev. Soc. Bras. Med. Trop.. 2020 Mar;53:e20190181. doi:10.1590/0037-8682-0181-2019.
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