Process intensification for production of Streptococcus pneumoniae whole-cell vaccine


Publication type
Article
Language
English
Appears in Collections:
Metrics
Abstract
The available pneumococcal conjugate vaccines provide protection against only those serotypes that are included in the vaccine, which leads to a selective pressure and serotype replacement in the population. An alternative low-cost, safe and serotype-independent vaccine was developed based on a nonencapsulated pneumococcus strain. This study evaluates process intensification to improve biomass production and shows for the first time the use of perfusion-batch with cell recycling for bacterial vaccine production. Batch, fed-batch, and perfusion-batch were performed at 10 L scale using a complex animal component-free culture medium. Cells were harvested at the highest optical density, concentrated and washed using microfiltration or centrifugation to compare cell separation methods. Higher biomass was achieved using perfusion-batch, which removes lactate while retaining cells. The biomass produced in perfusion-batch would represent at least a fourfold greater number of doses per cultivation than in the previously described batch process. Each strategy yielded similar vaccines in terms of quality as evaluated by western blot and animal immunization assays, indicating that so far, perfusion-batch is the best strategy for the intensification of pneumococcal whole-cell vaccine production, as it can be integrated to the cell separation process keeping the same vaccine quality.
Reference
Campos IB, Cardoso Junior CP, Fratelli F, Herd M, Moffitt KL., Lu Y-J, et al. Process intensification for production of Streptococcus pneumoniae whole-cell vaccine. Biotechnol. Bioeng.. 2020 Feb;:1-12. doi:10.1002/bit.27307.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/2983
Issue Date
2020


Files in This Item:

Existing users please Login
10.1002bit.27307.pdf
Size: 3.48 MB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show full item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.