Myriapod haemocyanin: the first three-dimensional reconstruction of Scolopendra subspinipes and preliminary structural analysis of S. viridicornis

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dc.contributorLaboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributor.authorRiciluca, Katie Cristina Takeutipt_BR
dc.contributor.authorBorges, A. C.pt_BR
dc.contributor.authorMello, J. F. R.pt_BR
dc.contributor.authorOliveira, Ursula Castro dept_BR
dc.contributor.authorSerdan, D. C.pt_BR
dc.contributor.authorFlorez-Ariza, A.pt_BR
dc.contributor.authorAguirre, Elisa Chaparropt_BR
dc.contributor.authorNishiyama Junior, Milton Yutakapt_BR
dc.contributor.authorCassago, A.pt_BR
dc.contributor.authorJunqueira-de-Azevedo, Inácio de Loiola Meirellespt_BR
dc.contributor.authorHeel, M. vanpt_BR
dc.contributor.authorSilva Junior, Pedro Ismael dapt_BR
dc.contributor.authorPortugal, R. V.pt_BR
dc.date.accessioned2020-07-09T21:27:14Z-
dc.date.available2020-07-09T21:27:14Z-
dc.date.issued2020-
dc.identifier.citationRiciluca KCT, Borges A.C., Mello J.F.R., Oliveira UC, Serdan D.C., Florez-Ariza A., et al. Myriapod haemocyanin: the first three-dimensional reconstruction of Scolopendra subspinipes and preliminary structural analysis of S. viridicornis. Open Biol.. 2020 Apr;10(4):190258. doi:10.1098/rsob.190258.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/2995-
dc.description.abstractHaemocyanins (Hcs) are copper-containing, respiratory proteins that occur in the haemolymph of many arthropod species. Here, we report the presence of Hcs in the chilopode Myriapoda, demonstrating that these proteins are more widespread among the Arthropoda than previously thought. The analysis of transcriptome of S. subspinipes subpinipes reveals the presence of two distinct subunits of Hc, where the signal peptide is present, and six of prophenoloxidase (PPO), where the signal peptide is absent, in the 75 kDa range. Size exclusion chromatography profiles indicate different quaternary organization for Hc of both species, which was corroborated by TEM analysis: S. viridicornis Hc is a 6 × 6-mer and S. subspinipes Hc is a 3 × 6-mer, which resembles the half-structure of the 6 × 6-mer but also includes the presence of phenoloxidases, since the 1 × 6-mer quaternary organization is commonly associated with hexamers of PPO. Studies with Chelicerata showed that PPO activity are exclusively associated with the Hcs. This study indicates that Scolopendra may have different proteins playing oxygen transport (Hc) and PO function, both following the hexameric oligomerization observed in Hcs.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent190258pt_BR
dc.languageengpt_BR
dc.relation.ispartofOpen Biologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleMyriapod haemocyaninpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1098/rsob.190258pt_BR
dc.identifier.urlhttps://doi.org/10.1098/rsob.190258pt_BR
dc.contributor.externalCentro Nacional de Pesquisa em Energia e Materiais (CNPEM)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.title.subthe first three-dimensional reconstruction of Scolopendra subspinipes and preliminary structural analysis of S. viridicornispt_BR
dc.identifier.citationvolume10pt_BR
dc.subject.keywordnegative stainpt_BR
dc.subject.keywordhaemocyaninpt_BR
dc.subject.keywordphenoloxidasept_BR
dc.subject.keywordmyriapodspt_BR
dc.subject.keywordcentipedespt_BR
dc.subject.keywordScolopendrapt_BR
dc.relation.ispartofabbreviatedOpen Biol.pt_BR
dc.identifier.citationabntv. 10, n. 4, 190258, abr. 2020pt_BR
dc.identifier.citationvancouver2020 Apr;10(4):190258pt_BR
dc.contributor.butantanRiciluca, Katie Cristina Takeuti|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:PrimeiroAutorpt_BR
dc.contributor.butantanJunqueira-de-Azevedo, Inácio de Loiola Meirelles|:Pesquisador:Docente Permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanOliveira, Ursula Castro de|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanAguirre, Elisa Chaparro|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanNishiyama Junior, Milton Yutaka|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.contributor.butantanSilva Junior, Pedro Ismael da|:Pesquisador|:Laboratório Especial de Toxinologia Aplicada (LETA)|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦472744/2012-7pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦232252/2014-9pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦400796/2012-0pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/15340-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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