Study of the polyribosyl-ribitol-phosphate precipitation mechanism by salts and organic solvents

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dc.contributor(LDP) Lab. Desenvolvimento de Processospt_BR
dc.contributor.authorSimas, Rodrigo Gabrielpt_BR
dc.contributor.authorTakagi, Mickiept_BR
dc.contributor.authorMiranda, Everson Alvespt_BR
dc.identifier.citationSimas RG, Takagi M, Miranda EA. Study of the polyribosyl-ribitol-phosphate precipitation mechanism by salts and organic solvents. Int. J. Biol. Macromol.. 2019 Nov;140:102-108. doi:10.1016/j.ijbiomac.2019.08.110.pt_BR
dc.description.abstractPrecipitation has been widely applied to purification and fractionation of biological macromolecules. Several physical-chemical factors contribute to the destabilization of those solutions, such as the nature of solvent employed, presence of salts, temperature, and concentration of the macromolecule. In the case of charged biopolymers, electrostatic forces are the major contributors to their stability in solution. However, the role of each variable and the exact mechanism of precipitation are not completely understood yet. The aim of this work was to study the precipitation of polyribosyl-ribitol-phosphate (PRP, a linear homogeneous anionic biopolymer) in presence of salts and non-solvents, in order to contribute to the elucidation of its precipitation mechanism. The solvents tested (acetone, ethanol, and isopropanol) presented distinct dielectric constants. The salts used (NH4Cl, NaCl, KCl, MgCl2, and CaCl2) differ by their cations. For each salt concentration, the solvent fraction that induces precipitation was identified and the dielectric constant of the bulk solution was calculated. Precipitation always occurred at well-defined combinations of solvents and salts. At low concentration of monovalent salts, there was a linear correlation between the logarithm of the salt concentration and the inverse of the medium dielectric constant at a defined precipitation point. This is a strong indication that the stability of the solution depends almost exclusively on the balance of electrostatic forces. This behavior is compatible with the DLVO modeling of colloidal systems. When divalent salts were used, low concentrations of the counterion were sufficient to induce precipitation, due to a phenomenon called ionic condensation. Apparently, PRP precipitates when around 90% of its charges are neutralized, value that is similar to charge neutralization for DNA precipitation.pt_BR
dc.description.sponsorship(BNDES) Banco Nacional de Desenvolvimento Econômico e Socialpt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.relation.ispartofInternational Journal of Biological Macromoleculespt_BR
dc.rightsRestricted accesspt_BR
dc.titleStudy of the polyribosyl-ribitol-phosphate precipitation mechanism by salts and organic solventspt_BR
dc.contributor.external(UNICAMP) Universidade Estadual de Campinaspt_BR
dc.subject.keywordIon condensationpt_BR
dc.relation.ispartofabbreviatedInt J Biol Macromolpt_BR
dc.identifier.citationabntv. 140, p. 102-108, nov. 2019pt_BR
dc.identifier.citationvancouver2019 Nov;140:102-108pt_BR
dc.contributor.butantanSimas, Rodrigo Gabriel|:Pesquisador|:Lab. Desenvolvimento de Processos|:PrimeiroAutorpt_BR
dc.contributor.butantanTakagi, Mickie|:Pesquisador|:Lab. Desenvolvimento de Processos|:pt_BR
dc.sponsorship.butantanBanco Nacional de Desenvolvimento Econômico e Social (BNDES)¦¦11.2.0322.1/2012pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
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