Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature

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dc.contributorLab. Herpetologiapt_BR
dc.contributor.authorTasima, Lidia Jorgept_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorHatakeyama, Daniela Mikipt_BR
dc.contributor.authorNishiduka, Erika S.pt_BR
dc.contributor.authorTashima, Alexandre K.pt_BR
dc.contributor.authorSant'Anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.date.accessioned2020-07-09T21:27:33Z-
dc.date.available2020-07-09T21:27:33Z-
dc.date.issued2020pt_BR
dc.identifier.citationTasima LJ, Silva CS, Hatakeyama DM, Nishiduka ES., Tashima AK., Sant'Anna SS, et al. Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature. J. Venom. Anim. Toxins Incl. Trop. Dis.. 2020 Apr;26:e20190053. doi:10.1590/1678-9199-jvatitd-2019-0053.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3019-
dc.description.abstractBackground: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy. Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and -negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected. Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extente20190053pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleCrotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or naturept_BR
dc.typeArticlept_BR
dc.identifier.doi10.1590/1678-9199-jvatitd-2019-0053pt_BR
dc.identifier.urlhttps://doi.org/10.1590/1678-9199-jvatitd-2019-0053pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume26pt_BR
dc.subject.keywordRattlesnakept_BR
dc.subject.keywordSnake venompt_BR
dc.subject.keywordToxinspt_BR
dc.subject.keywordVenom variationpt_BR
dc.subject.keywordAntivenompt_BR
dc.relation.ispartofabbreviatedJ Venom Anim Toxins Incl Trop Dispt_BR
dc.identifier.citationabntv. 26, e20190053, abr. 2020pt_BR
dc.identifier.citationvancouver2020 Apr;26:e20190053pt_BR
dc.contributor.butantanTasima, Lidia Jorge|:Aluno|:Lab. Herpetologia|:PrimeiroAutorpt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanHatakeyama, Daniela Miki|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanSant'Anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/11108-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/03839-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/03311-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/20106-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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