Immunization with PhtD truncated fragments reduces nasopharyngeal colonization by Streptococcus pneumoniae

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dc.contributor(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorAndre, Greiciely O.pt_BR
dc.contributor.authorAssoni, Lucaspt_BR
dc.contributor.authorRodríguez, Duniapt_BR
dc.contributor.authorLeite, Luciana Cezar de Cerqueirapt_BR
dc.contributor.authorSantos, Thaisy E.P. dospt_BR
dc.contributor.authorFerraz, Lucio F.C.pt_BR
dc.contributor.authorConverso, Thiago Rojaspt_BR
dc.contributor.authorDarrieux, Michellept_BR
dc.date.accessioned2020-07-09T21:27:35Z-
dc.date.available2020-07-09T21:27:35Z-
dc.date.issued2020pt_BR
dc.identifier.citationAndre GO., Assoni L, Rodríguez D, Leite LCC, Santos TE.P., Ferraz LF.C., et al. Immunization with PhtD truncated fragments reduces nasopharyngeal colonization by Streptococcus pneumoniae. Vaccine. 2020 Apr;38(26):4146-4153. doi:10.1016/j.vaccine.2020.04.050.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3022-
dc.description.abstractDespite the undeniable success of polysaccharide vaccines against Streptococcus pneumoniae infections, there is a consensus on the scientific field that this approach should be revised in order to overpass the problems related with these formulations, such as serotype replacement and high production costs. The study of conserved pneumococcal proteins or its truncated fragments has emerged as a serotype independent alternative. In this work, we have characterized the immune response elicited by systemic immunization of mice with the Histidine triad protein D (PhtD) and its’ amino and carboxyl terminal fragments. The proteins were shown to be immunogenic and protective against pneumococcal colonization, with increased IL-17 production, and induction of antibodies able to limit pneumococcal adhesion to human respiratory cells. Antiserum against PhtD_Nter, but not C_ter or PhtD, promoted an increase in bacterial phagocytosis in vitro. Interestingly, antibodies against the PhtD_Nter displayed cross-reactivity with two other pneumococcal proteins, PspA and PspC, due to sequence similarities in the proline rich region of the molecules. On a whole, our results support the inclusion of PhtD, and more specifically, its N-terminal fragment, in a multicomponent serotype independent vaccinept_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.extent4146-4153pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofVaccinept_BR
dc.rightsRestricted accesspt_BR
dc.titleImmunization with PhtD truncated fragments reduces nasopharyngeal colonization by Streptococcus pneumoniaept_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.vaccine.2020.04.050pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.vaccine.2020.04.050pt_BR
dc.contributor.external(USF) Universidade São Franciscopt_BR
dc.identifier.citationvolume38pt_BR
dc.identifier.citationissue26pt_BR
dc.subject.keywordPneumococcal vaccinept_BR
dc.subject.keywordPhtDpt_BR
dc.subject.keywordColonizationpt_BR
dc.subject.keywordStreptococcus pneumoniaept_BR
dc.relation.ispartofabbreviatedVaccinept_BR
dc.identifier.citationabntv. 38, n. 26, p. 4146-4153, abr. 2020pt_BR
dc.identifier.citationvancouver2020 Apr;38(26):4146-4153pt_BR
dc.contributor.butantanRodríguez, Dunia Del Carmen|:Pesquisador|:(LDV) Lab. Desenvolvimento de Vacinas|:pt_BR
dc.contributor.butantanLeite, Luciana Cezar de Cerqueira|:Pesquisador|:(LDV) Lab. Desenvolvimento de Vacinas|:pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦PROSUC 88887.160030/2017-00pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/07249-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.languageiso639-1English-
item.grantfulltextembargo_29990101-
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