Comparative analysis of the minimum number of replication origins in trypanosomatids and yeasts

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dc.contributorLCC - Laboratório de Ciclo Celularpt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributor.authorSilva, Marcelo Santos dapt_BR
dc.contributor.authorVitarelli, Marcela de Oliveirapt_BR
dc.contributor.authorSouza, Bruno Ferreira dept_BR
dc.contributor.authorElias, Maria Carolinapt_BR
dc.date.accessioned2020-07-09T21:27:47Z-
dc.date.available2020-07-09T21:27:47Z-
dc.date.issued2020pt_BR
dc.identifier.citationSilva MS, Vitarelli MO, Souza BF, Elias MC. Comparative analysis of the minimum number of replication origins in trypanosomatids and yeasts. Genes. 2020 May;11(5):523. doi:10.3390/genes11050523.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3041-
dc.description.abstractSingle-celled eukaryote genomes predominantly replicate through multiple origins. Although origin usage during the S-phase has been elucidated in some of these organisms, few studies have comparatively approached this dynamic. Here, we developed a user-friendly website able to calculate the length of the cell cycle phases for any organism. Next, using a formula developed by our group, we showed a comparative analysis among the minimum number of replication origins (MO) required to duplicate an entire chromosome within the S-phase duration in trypanosomatids (Trypanosoma cruzi, Leishmania major, and Trypanosoma brucei) and yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe). Using the data obtained by our analysis, it was possible to predict the MO required in a situation of replication stress. Also, our findings allow establishing a threshold for the number of origins, which serves as a parameter for genome approaches that map origins. Moreover, our data suggest that when compared to yeasts, trypanosomatids use much more origins than the minimum needed. This is the first time a comparative analysis of the minimum number of origins has been successfully applied. These data may provide new insight into the understanding of the replication mechanism and a new methodological framework for studying single-celled eukaryote genomes.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent523pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofGenespt_BR
dc.rightsOpen Accesspt_BR
dc.titleComparative analysis of the minimum number of replication origins in trypanosomatids and yeastspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3390/genes11050523pt_BR
dc.identifier.urlhttps://doi.org/10.3390/genes11050523pt_BR
dc.identifier.citationvolume11pt_BR
dc.identifier.citationissue5pt_BR
dc.subject.keywordtrypanosomatidspt_BR
dc.subject.keywordyeastspt_BR
dc.subject.keywordtrypanosomatids genomept_BR
dc.subject.keywordcell cycle phasespt_BR
dc.subject.keywordS-phase durationpt_BR
dc.subject.keywordDNA replicationpt_BR
dc.subject.keywordreplication originspt_BR
dc.relation.ispartofabbreviatedGenespt_BR
dc.identifier.citationabntv. 11, n. 5, 523, mai. 2020pt_BR
dc.identifier.citationvancouver2020 May;11(5):523pt_BR
dc.contributor.butantanSilva, Marcelo Santos da|:Aluno|:LCC - Laboratório de Ciclo Celular|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanVitarelli, Marcela de Oliveira|:Aluno|:LCC - Laboratório de Ciclo Celular|:pt_BR
dc.contributor.butantanSouza, Bruno Ferreira de|:Aluno|:Laboratório de Toxinologia Aplicada|:pt_BR
dc.contributor.butantanElias, Maria Carolina|:Pesquisador|:LCC - Laboratório de Ciclo Celular|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦306199/2018-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦870219/1997-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/50050-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/24170-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/18719-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/07693-2pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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item.languageiso639-1English-
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