H7N9 influenza split vaccine with SWE oil-in-water adjuvant greatly enhances cross-reactive humoral immunity and protection against severe pneumonia in ferrets

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dc.contributorSeção de Vacinas Aeróbicaspt_BR
dc.contributorCentro Bioindustrialpt_BR
dc.contributor.authorJonge, Jørgen dept_BR
dc.contributor.authorDijken, Harry vanpt_BR
dc.contributor.authorHeij, Femke dept_BR
dc.contributor.authorSpijkers, Sannept_BR
dc.contributor.authorMouthaan, Justinpt_BR
dc.contributor.authorJong, Rineke dept_BR
dc.contributor.authorRoholl, Paulpt_BR
dc.contributor.authorAdami, Eduardo Alfredopt_BR
dc.contributor.authorAkamatsu, Milena Apetitopt_BR
dc.contributor.authorHo, Paulo Leept_BR
dc.contributor.authorBrunner, Liviapt_BR
dc.contributor.authorCollin, Nicolaspt_BR
dc.contributor.authorFriede, Martinpt_BR
dc.contributor.authorFerreira, José A.pt_BR
dc.contributor.authorLuytjes, Willempt_BR
dc.date.accessioned2020-07-09T21:27:50Z-
dc.date.available2020-07-09T21:27:50Z-
dc.date.issued2020pt_BR
dc.identifier.citationJonge J, Dijken H, Heij F, Spijkers S, Mouthaan J, Jong R, et al. H7N9 influenza split vaccine with SWE oil-in-water adjuvant greatly enhances cross-reactive humoral immunity and protection against severe pneumonia in ferrets. NPJ Vaccines. 2020 May;5:38. doi:10.1038/s41541-020-0187-4.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3044-
dc.description.abstractUntil universal influenza vaccines become available, pandemic preparedness should include developing classical vaccines against potential pandemic influenza subtypes. We here show that addition of SWE adjuvant, a squalene-in-water emulsion, to H7N9 split influenza vaccine clearly enhanced functional antibody responses in ferrets. These were cross-reactive against H7N9 strains from different lineages and newly emerged H7N9 variants. Both vaccine formulations protected in almost all cases against severe pneumonia induced by intratracheal infection of ferrets with H7N9 influenza; however, the SWE adjuvant enhanced protection against virus replication and disease. Correlation analysis and curve fitting showed that both VN- and NI-titers were better predictors for protection than HI-titers. Moreover, we show that novel algorithms can assist in better interpretation of large data sets generated in preclinical studies. Cluster analysis showed that the adjuvanted vaccine results in robust immunity and protection, whereas the response to the non-adjuvanted vaccine is heterogeneous, such that the protection balance may be more easily tipped toward severe disease. Finally, cluster analysis indicated that the dose-sparing capacity of the adjuvant is at least a factor six, which greatly increases vaccine availability in a pandemic situation.pt_BR
dc.description.sponsorshipWorld Health Organization (WHO)pt_BR
dc.description.sponsorshipBiomedical Advanced Research and Development Authority (BARDA)pt_BR
dc.description.sponsorshipGerman Technical Cooperationpt_BR
dc.format.extent38pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofNPJ Vaccinespt_BR
dc.rightsOpen Accesspt_BR
dc.titleH7N9 influenza split vaccine with SWE oil-in-water adjuvant greatly enhances cross-reactive humoral immunity and protection against severe pneumonia in ferretspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1038/s41541-020-0187-4pt_BR
dc.identifier.urlhttps://doi.org/10.1038/s41541-020-0187-4pt_BR
dc.contributor.externalNetherlands National Institute for Public Health and the Environment (RIVM)¦¦Holandapt_BR
dc.contributor.externalCentral Veterinary Institute of Wageningen UR¦¦Holandapt_BR
dc.contributor.externalMicroscope Consultancy¦¦Holandapt_BR
dc.contributor.externalUniversité de Lausanne (UNIL)¦¦Suíçapt_BR
dc.contributor.externalWorld Health Organization Switzerland (WHO)¦¦Suiçapt_BR
dc.identifier.citationvolume5pt_BR
dc.subject.keywordAdaptive immunitypt_BR
dc.subject.keywordInfectionpt_BR
dc.subject.keywordInfectious diseasespt_BR
dc.subject.keywordVaccinespt_BR
dc.relation.ispartofabbreviatedNPJ Vaccinespt_BR
dc.identifier.citationabntv. 5, 38, mai. 2020pt_BR
dc.identifier.citationvancouver2020 May;5:38pt_BR
dc.contributor.butantanAdami, Eduardo Alfredo|:Técnico|:Seção de Vacinas Aeróbicas|:pt_BR
dc.contributor.butantanAkamatsu, Milena Apetito|:Pesquisador|:Seção de Vacinas Aeróbicas|:pt_BR
dc.contributor.butantanHo, Paulo Lee|:Pesquisador|:Centro Bioindustrial|:pt_BR
dc.sponsorship.butantanBiomedical Advanced Research and Development Authority (BARDA)¦¦1-IDSEP100009-01-00pt_BR
dc.sponsorship.butantanGerman Technical Cooperation¦¦81134450pt_BR
dc.sponsorship.butantanWorld Health Organization (WHO)¦¦2013/342684pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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