Proteomic analysis of Trypanosoma cruzi spliceosome complex


Publication type
Article
Appears in Collections:
Metrics
Abstract
The unicellular protists of the group Kinetoplastida include the genera Leishmania and Trypanosoma, which are pathogens of invertebrate and vertebrate animals. Despite their medical and economical importance, critical aspects of their biology such as specific molecular characteristics of gene expression regulation are just beginning to be deciphered. Gene expression regulation also depends on post-transcriptional processing steps, such as the trans-splicing process. Despite being widely used in trypanosomes, trans-splicing is a rare event in other eukaryotes. We sought to describe the protein composition of spliceosomes in epimastigotes of T. cruzi, the etiological agent of Chagas disease. We used two TAP-tagged proteins to affinity purify spliceosomes and analyzed their composition by mass spectrometry. Among the 115 identified proteins we detected conserved spliceosome components, as Sm and LSm proteins, RNA helicases, U2- and U5-snRNP specific proteins. Importantly, by comparing our data with proteomic data of human and T. brucei spliceosome complexes, we observed a core group of proteins common to all spliceosomes. By using amino acid sequence comparisons, we identified RNA-associated proteins that might be involved with splicing regulation in T. cruzi, namely the orthologous of WDR33, PABPCL1 and three different HNRNPs. Data are available via ProteomeXchange with identifier PXD018776.
Reference
Barbosa RL., da Cunha JPC, Menezes AT., Melo RF.P., Elias MC, Silber AM., et al. Proteomic analysis of Trypanosoma cruzi spliceosome complex. J. Proteomics. 2020 Jul;223:103822. doi:10.1016/j.jprot.2020.103822.
Link to cite this reference
https://repositorio.butantan.gov.br/handle/butantan/3045
Journal title
Issue Date
2020


Files in This Item:

Existing users please Login
10.1016j.jprot.2020.103822.pdf
Size: 410.34 kB
Format: Adobe PDF
Embargoed until January 1, 2999    Request a copy
Show full item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.