Effect of Tityus serrulatus scorpion venom on isolated jejunum: a very useful tool to study the interaction between neurons in the enteric nervous system

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dc.contributorLab. Farmacologiapt_BR
dc.contributorUnidade de Modo de Ação de Toxinaspt_BR
dc.contributor.authorLouza, Gisele Siquelli Gouveiapt_BR
dc.contributor.authorCarmo, Lúcia Lameirão Garcez dopt_BR
dc.contributor.authorConceição, Isaltino Marcelopt_BR
dc.date.accessioned2020-07-09T21:27:56Z-
dc.date.available2020-07-09T21:27:56Z-
dc.date.issued2020pt_BR
dc.identifier.citationLouza GSG, Carmo LLG, Conceição IM. Effect of Tityus serrulatus scorpion venom on isolated jejunum: a very useful tool to study the interaction between neurons in the enteric nervous system. Auton. Neurosci.. 2020 Sep;227:102676. doi:10.1016/j.autneu.2020.102676.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3053-
dc.description.abstractScorpion envenomation is a public health problem in tropical and subtropical areas. In Brazil, Tityus serrulatus is the biggest cause of accidents with venomous animals. Tityus serrulatus venom causes symptoms related to a great activation of the autonomic system attributed to a massive release of sympathetic and parasympathetic mediators. This effect is attributed to the presence of toxins acting in Na+ and K+ ion channels, leading to an increase in cell excitability. Although gastrointestinal symptoms, like diarrhoea and sialorrhea, is observed in moderate to severe cases, little attention is given in clinical reports. Gastrointestinal motility is controlled by the enteric nervous system which is composed of a wide variety of interconnected neurons that are influenced by the sympathetic and parasympathetic nervous systems. Thus, this work aimed to characterize the effects of Tityus serrulatus venom on sympathetic and parasympathetic neurotransmission of rat jejunum, as well as to investigate possibles effects on other neurons of the enteric nervous system. To this, we verify the effects of Tityus serrulatus venom on the contractility of isolated rat jejunum through organ-bath experiments. We observed that venom can induce both contraction and relaxation. The contraction was partially inhibited by atropine (1 µM) and by suramin (0.1 mM) through tetrodotoxin-resistant and sensitive mechanisms. The relaxation was completely inhibited by 3 µM propranolol and partially inhibited by 1 µM phentolamine. Suramin induced a slowing of relaxation curve. Tetrodotoxin completely inhibits the relaxation induced by Tityus serrulatus venom, but the contraction curves were only partially reduced in their initial portion. The final part of the curve was largely enhanced by Tetrodotoxin. Atropine blocks almost completely the contraction curve in the presence of Tetrodotoxin. These results indicate that Tityus serrulatus venom induces the release of both excitatory (predominantly acetylcholine) and inhibitory (mainly noradrenaline) neurotransmitters. The effects of Tityus serrulatus venom on organ contractility was quite complex and seem to derive from a diffuse and nonspecific release of mediators from autonomic and enteric nervous systems. Further investigation of venom action and its isolated toxins can reveal important aspects to deepen our knowledge about the enteric nervous system transmission and the interaction between excitatory and inhibitory mediators as well as the physiological role of Na+ and K+ ion channels in gut motility.pt_BR
dc.format.extent102676pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofAutonomic Neurosciencept_BR
dc.titleEffect of Tityus serrulatus scorpion venom on isolated jejunum: a very useful tool to study the interaction between neurons in the enteric nervous systempt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.autneu.2020.102676pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.autneu.2020.102676pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume227pt_BR
dc.subject.keywordEnteric nervous systempt_BR
dc.subject.keywordTityus serrulatus venompt_BR
dc.subject.keywordAutonomic nervous systempt_BR
dc.subject.keywordNeurotransmissionpt_BR
dc.subject.keywordNa+ channelpt_BR
dc.relation.ispartofabbreviatedAuton Neuroscipt_BR
dc.identifier.citationabntv. 227, 102676, set. 2020pt_BR
dc.identifier.citationvancouver2020 Sep;227:102676pt_BR
dc.contributor.butantanGisele Siquelli Gouveia Louza|:Aluno|:Lab. Farmacologia|:PrimeiroAutorpt_BR
dc.contributor.butantanConceição, Isaltino Marcelo|:Pesquisador|:Lab. Farmacologia:Unidade de Modo de Ação de Toxinas|:Autor de correspondênciapt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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crisitem.author.orcid0000-0003-2392-8103-
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