Comparative analysis of rapid agglutination latex test using single-chain antibody fragments (scFv) versus the gold standard vero cell assay for Shiga toxin (Stx) detection

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorShiga, Emerson Andradept_BR
dc.contributor.authorGuth, B.E.C.pt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorLuz, Danielapt_BR
dc.date.accessioned2020-07-09T21:28:01Z-
dc.date.available2020-07-09T21:28:01Z-
dc.date.issued2020pt_BR
dc.identifier.citationShiga EA, Guth B.E.C., Piazza RMF, Luz D. Comparative analysis of rapid agglutination latex test using single-chain antibody fragments (scFv) versus the gold standard vero cell assay for Shiga toxin (Stx) detection. J. Microbiol. Methods. 2020 Jun;175:105965. doi:10.1016/j.mimet.2020.105965.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3059-
dc.description.abstractThe latex agglutination test using single-chain antibody fragments (scFvStx1 and scFvStx2) coupled to latex particles, was compared with the gold standard Vero cell assay for Shiga toxin (Stx) detection, aiming to estimate the diagnosis potential of these scFv fragments in a rapid and straightforward test. The latex complexes identified the presence of the toxins up to a 1:8 dilution in the majority of the evaluated strains. Moreover, the Stx concentration was indirectly determined in Stx-producing Escherichia coli (STEC) strains, allowing detection limit inference. A Stx dilution curve was constructed, and the data was analyzed in a non-linear model by second-order polynomial regression for prediction (p-value of 0.001 and a R2 above 0.98 were considered for correlations). The detection limit was 30ng/mL for Stx1 and 10ng/mL for Stx2. The scFvStx1 and scFvStx2 coupled to latex nanoparticles provide a toxin assay with a competitive Stx detection limit, which has a low cost and short execution time. The diagnostic method proposed here, using, for the first time, recombinant antibody fragments, raises the possibility of developing a more affordable test to be used in the routine detection and surveillance of STEC infections.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent105965pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Microbiological Methodspt_BR
dc.titleComparative analysis of rapid agglutination latex test using single-chain antibody fragments (scFv) versus the gold standard vero cell assay for Shiga toxin (Stx) detectionpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.mimet.2020.105965pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.mimet.2020.105965pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)¦¦Brasilpt_BR
dc.identifier.citationvolume175pt_BR
dc.subject.keywordRALTpt_BR
dc.subject.keywordSTECpt_BR
dc.subject.keywordDiagnosispt_BR
dc.subject.keywordShiga toxinpt_BR
dc.relation.ispartofabbreviatedJ Microbiol Methodspt_BR
dc.identifier.citationabntv. 175, 105965, jun. 2020pt_BR
dc.identifier.citationvancouver2020 Jun;175:105965pt_BR
dc.contributor.butantanShiga, Emerson Andrade|:Aluno|:Lab. Bacteriologia|:PrimeiroAutorpt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador:Docente Permanente PPGTOX|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.contributor.butantanLuz, Daniela|:Aluno|:Lab. Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦2010/201484pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦2013/03160-9pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦PNPD20132071pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2015/17178-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17006-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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