Sexual and ontogenetic variation of Bothrops leucurus venom

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dc.contributorLab. Herpetologiapt_BR
dc.contributorCurso de Especialização em Toxinas de Interesse em Saúdept_BR
dc.contributor.authorBraga, Jacqueline Ramos Machadopt_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.contributor.authorPereira, Diego dos Santospt_BR
dc.contributor.authorSant'Anna, Sávio Stefaninipt_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorVilarinho, Ariel Rodrigues Gomespt_BR
dc.contributor.authorRodrigues, José Luccapt_BR
dc.contributor.authorRocha, Marisa Maria Teixeira dapt_BR
dc.date.accessioned2020-07-09T21:28:12Z-
dc.date.available2020-07-09T21:28:12Z-
dc.date.issued2020pt_BR
dc.identifier.citationBraga JRM, Morais-Zani K, Pereira DS, Sant'Anna SS, Galizio NC, Tanaka-Azevedo AM, et al. Sexual and ontogenetic variation of Bothrops leucurus venom. Toxicon. 2020 Sep;184:127-135. doi:10.1016/j.toxicon.2020.05.028.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3073-
dc.description.abstractVarious factors, such as geographical origin, climate, sex, age and diet can influence the composition and pathophysiological activities of snake venoms. In this study, we examined the sexual and ontogenetic variations in the venom of Bothrops leucurus, a pitviper responsible for more than 80% of the snakebites in the state of Bahia, northeastern Brazilian. The venoms of 31 snakes were pooled according to sex and age (young, adult and old) and screened by SDS-PAGE (in reducing and non-reducing conditions), reverse-phase high performance liquid chromatography (RP-HPLC), gelatin zymography, and immunoblotting with therapeutic bothropic antivenom (BAV) from the Instituto Butantan. The electrophoretic and chromatographic profiles showed intraspecific ontogenetic variation, whereas sexual variations were less evident. All venoms showed gelatinolytic activity associated with 50–75 kDa protein bands. In addition, all venoms, regardless of the snakes' sex and age, cross-reacted to similar extents with BAV. Our findings show that B. leucurus venom changes during ontogenetic development and demonstrate sexual differences in its composition, indicating differences in biological activity.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent127-135pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxiconpt_BR
dc.titleSexual and ontogenetic variation of Bothrops leucurus venompt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.toxicon.2020.05.028pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.toxicon.2020.05.028pt_BR
dc.contributor.externalUniversidade Federal do Recôncavo da Bahia (UFRB)¦¦Brasilpt_BR
dc.contributor.externalUniversidade de São Paulo (USP)¦¦Brasilpt_BR
dc.identifier.citationvolume184pt_BR
dc.subject.keywordAgept_BR
dc.subject.keywordBothrops leucuruspt_BR
dc.subject.keywordSexpt_BR
dc.subject.keywordSnake venompt_BR
dc.subject.keywordVariabilitypt_BR
dc.relation.ispartofabbreviatedToxiconpt_BR
dc.identifier.citationabntv. 184, p. 127-135, set. 2020pt_BR
dc.identifier.citationvancouver2020 Sep;184:127-135pt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanSant'Anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:pt_BR
dc.contributor.butantanVilarinho, Ariel Rodrigues Gomes|:Aluno|:Curso de Especialização em Toxinas de Interesse em Saúde|:pt_BR
dc.contributor.butantanRodrigues, José Lucca|:Aluno|:Curso de Especialização em Toxinas de Interesse em Saúde|:pt_BR
dc.contributor.butantanRocha, Marisa Maria Teixeira da|:Técnico|:Lab. Herpetologia|:pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦405399/2018–9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/11108–0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/09976–9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908–2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/14724–4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786–0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
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item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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