Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom

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dc.contributorLab. Herpetologiapt_BR
dc.contributor.authorTasima, Lidia Jorgept_BR
dc.contributor.authorHatakeyama, Daniela Mikipt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorFabri, Carolinept_BR
dc.contributor.authorLima, Eduardo Oliveira Venâncio dept_BR
dc.contributor.authorSant'anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.contributor.authorSanz, Libiapt_BR
dc.contributor.authorCalvete, Juan J.pt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.date.accessioned2020-08-03T18:35:13Z-
dc.date.available2020-08-03T18:35:13Z-
dc.date.issued2020pt_BR
dc.identifier.citationTasima LJ, Hatakeyama DM, Silva CS, Fabri C, Lima EOV, Sant'anna SS, et al. Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom. Toxicon. 2020 Oct;185:26-35. doi:10.1016/j.toxicon.2020.07.001.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3093-
dc.description.abstractThe South American rattlesnake Crotalus durissus spp has a wide geographic distribution in Brazil. Although responsible for only a low proportion of ophidian accidents, it is considered one of the most medically important species of venomous snakes due to the high mortality rate (1.87%). Snake venom is a complex phenotype commonly subjected to individual intraspecific, ontogenetic and geographic variability. Compositional differences in pooled venom used in the immunization process may impact the efficacy of the antivenom. In order to assure standardized high-quality antivenom, the potency of each Brazilian crotalic antivenom batch is determined against the ‘Brazilian Crotalic Reference Venom’ (BCRV). BCRV is produced by Instituto Butantan using venom obtained from the first milking of recently wild-caught C. d. terrificus specimens brought to the Institute. The decrease in the number of snake donations experienced in recent years can become a threat to the production of future batches of BCRV. To evaluate the feasibility of using venom from long-term captive animals in the formulation of BCRV, we have compared the proteomic, biochemical and biological profiles of C. d. terrificus venom pooled from captive specimens (CVP- captive venom pool) and BCRV. Electrophoretic and venomics analyses revealed a very similar venom composition profile, but also certain differences in toxins abundance, with some low abundant protein families found only in BCRV. Enzymatic (L-amino acid oxidase, phospholipase A2 and proteolytic) and biological (myotoxic and coagulant) activities showed higher values in CVP than in BCRV. CVP also possessed slightly higher lethal effect, although the Instituto Butantan crotalic antivenom showed equivalent potency neutralizing BCRV and CVP. Our results strongly suggest that venom from long-term captive C. d. terrificus might be a valid alternative to generate an immunization mixture of equivalent quality to the currently in use reference venom.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidadespt_BR
dc.format.extent26-35pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxiconpt_BR
dc.titleComparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venompt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.toxicon.2020.07.001pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.toxicon.2020.07.001pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalConsejo Superior de Investigaciones Cientificas (CSIC)pt_BR
dc.identifier.citationvolume185pt_BR
dc.subject.keywordCrotalus durissus terrificus venompt_BR
dc.subject.keywordSnake venomicspt_BR
dc.subject.keywordVenom of wild caught versus captive snakept_BR
dc.subject.keywordBrazilian crotalic reference venompt_BR
dc.relation.ispartofabbreviatedToxiconpt_BR
dc.identifier.citationabntv. 185, p. 26-35, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;185:26-35pt_BR
dc.contributor.butantanTasima, Lidia Jorge|:Aluno|:Lab. Herpetologia|:PrimeiroAutorpt_BR
dc.contributor.butantanHatakeyama, Daniela Miki|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanFabri, Caroline|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanLima, Eduardo Oliveira Venâncio de|:Aluno|:Lab. Herpetologiapt_BR
dc.contributor.butantanSant'anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanMorais-Zani, Karen de|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890–0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786–0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25899–0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/26533–6pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908–2pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦405399/2018–9pt_BR
dc.sponsorship.butantanMinisterio de Ciencia, Innovación y Universidades¦¦BFU2017-89103-Ppt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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