Heterologous expression of the pathogen-specific LIC11711 gene in the Saprophyte L. biflexa increases bacterial binding to laminin and plasminogen

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Campo DCValoridioma
dc.contributor(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorKochi, Leandro Toshiopt_BR
dc.contributor.authorFernandes, Luis Guilherme Vírgíliopt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.date.accessioned2020-08-05T18:47:04Z-
dc.date.available2020-08-05T18:47:04Z-
dc.date.issued2020pt_BR
dc.identifier.citationKochi LT, Fernandes LGV, Nascimento ALTO. Heterologous expression of the pathogen-specific LIC11711 gene in the Saprophyte L. biflexa increases bacterial binding to laminin and plasminogen. Pathogens. 2020 Jul;9(8):599. doi:10.3390/pathogens9080599.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3114-
dc.description.abstractLeptospirosis is a febrile disease and the etiological agents are pathogenic bacteria of the genus Leptospira. The leptospiral virulence mechanisms are not fully understood and the application of genetic tools is still limited, despite advances in molecular biology techniques. The leptospiral recombinant protein LIC11711 has shown interaction with several host components, indicating a potential function in virulence. This study describes a system for heterologous expression of the L. interrogans gene lic11711 using the saprophyte L. biflexa serovar Patoc as a surrogate, aiming to investigate its possible activity in bacterial virulence. Heterologous expression of LIC11711 was performed using the pMaOri vector under regulation of the lipL32 promoter. The protein was found mainly on the leptospiral outer surface, confirming its location. The lipL32 promoter enhanced the expression of LIC11711 in L. biflexa compared to the pathogenic strain, indicating that this strategy may be used to overexpress low-copy proteins. The presence of LIC11711 enhanced the capacity of L. biflexa to adhere to laminin (Lam) and plasminogen (Plg)/plasmin (Pla) in vitro, suggesting the involvement of this protein in bacterial pathogenesis. We show for the first time that the expression of LIC11711 protein of L. interrogans confers a virulence-associated phenotype on L. biflexa, pointing out possible mechanisms used by pathogenic leptospires.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent599pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPathogenspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleHeterologous expression of the pathogen-specific LIC11711 gene in the Saprophyte L. biflexa increases bacterial binding to laminin and plasminogenpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/pathogens9080599pt_BR
dc.identifier.urlhttps://doi.org/10.3390/pathogens9080599pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume9pt_BR
dc.identifier.citationissue8pt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordleptospirosispt_BR
dc.subject.keywordvirulencept_BR
dc.relation.ispartofabbreviatedPathogenspt_BR
dc.identifier.citationabntv. 9, n. 8, 599, jul. 2020pt_BR
dc.identifier.citationvancouver2020 Jul;9(8):599pt_BR
dc.contributor.butantanKochi, Leandro Toshio|:Aluno|:(LDV) Lab. Desenvolvimento de Vacinas|:PrimeiroAutorpt_BR
dc.contributor.butantanFernandes, Luis Guilherme Vírgílio|:Aluno|:(LDV) Lab. Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:(LDV) Lab. Desenvolvimento de Vacinas|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦14/50981-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/09652-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/06731-8pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)¦¦301229/2017-1pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.grantfulltextopen-
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crisitem.author.orcid0000-0003-4851-0870-
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