Contribution of complement system pathways to the killing of leptospira spp.

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorSilva, Priscilla Yuri Okochi Alves dapt_BR
dc.contributor.authorMidon, Leonardo Mourapt_BR
dc.contributor.authorHeinemann, Marcos Bryanpt_BR
dc.contributor.authorVasconcelos, Dewton de Moraespt_BR
dc.contributor.authorBarbosa, Angela Silvapt_BR
dc.contributor.authorIsaac, Lourdespt_BR
dc.date.accessioned2020-08-06T19:04:06Z-
dc.date.available2020-08-06T19:04:06Z-
dc.date.issued2020pt_BR
dc.identifier.citationSilva PYOA, Midon LM, Heinemann MB, Vasconcelos DM, Barbosa AS, Isaac L. Contribution of complement system pathways to the killing of leptospira spp.. Microbes Infect.. 2020 Dec;22(10):550-557. doi:10.1016/j.micinf.2020.07.005.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3118-
dc.description.abstractThe Complement System CS plays an important role in the immune response against leptospirosis and can be activated by the Alternative and Lectin Pathways (Innate Immunity) and by the Classical Pathway (Acquired Immunity). Here we analyzed a broad range of nonpathogenic and pathogenic Leptospira strains considering their interaction with each CS pathway. We determined bacterial survival rate and CS protein deposition in the presence of purified proteins, specific component depleted sera and NHS treated with the chelating agents EDTA inhibits all three activation pathways) or EGTA inhibits the Classical and Lectin Pathways. We suggest that the Lectin and the Alternative Pathways have an important role to eliminate saprophytic leptospires since i) approximately 50% survival of both saprophytic strains was observed in the presence of MBL-deficient serum; ii) approximately 50 % survival of L. biflexa Patoc I was observed in the presence of NHS – EGTA and iii) C1q-depleted serum caused significant bacterial lysis. In all serovars investigated the deposition of C5-C9 proteins on saprophytic Leptospira strains was more pronounced when compared to pathogenic species confirming previous studies in the literature. No difference on C3 deposition was observed between nonpathogenic and pathogenic strains. In conclusion, Leptospira strains interact to different degrees with CS proteins, especially those necessary to form MAC, indicating that some strains and specific ligands could favor the binding of certain CS proteins.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent550-557pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofMicrobes and Infectionpt_BR
dc.titleContribution of complement system pathways to the killing of leptospira spp.pt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.micinf.2020.07.005pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.micinf.2020.07.005pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume22pt_BR
dc.identifier.citationissue10pt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordComplement System. Immune evasionpt_BR
dc.relation.ispartofabbreviatedMicrobes Infect.pt_BR
dc.identifier.citationabntv. 22, n. 10, p. 550-557, jul. 2020pt_BR
dc.identifier.citationvancouver2020 Dec;22(10):550-557pt_BR
dc.contributor.butantanBarbosa, Angela Silva|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/12924-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/18936-3pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦307780/2017-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦305114/2017-4pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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