Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice

Full metadata record
DC FieldValueLanguage
dc.contributorLab. Fisiopatologiapt_BR
dc.contributorLab. Imunogenéticapt_BR
dc.contributor.authorSachetto, Ana Teresa Azevedopt_BR
dc.contributor.authorJensen, José Ricardopt_BR
dc.contributor.authorSantoro, Marcelo Laramipt_BR
dc.date.accessioned2020-08-27T18:51:06Z-
dc.date.available2020-08-27T18:51:06Z-
dc.date.issued2020pt_BR
dc.identifier.citationSachetto ATA, Jensen JR, Santoro ML. Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice. PLoS. Negl. Trop. Dis.. 2020 June;14(6):e0008379. doi:10.1371/journal.pntd.0008379.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3145-
dc.description.abstractFew studies have addressed gene expression of hemostasis-related factors during acute thrombo-hemorrhagic diseases. Bites by the lanced-headed viper Bothrops jaracaca induce rapid hemostatic disturbances in victims, leading to systemic bleedings, thrombocytopenia and consumption coagulopathy. Although circulating levels of coagulation factors recover rapidly after administration of specific antivenom therapy, it is unclear if B. jararaca venom (BjV) upregulates the mRNA synthesis of hepatic hemostasis-related factors, or if the recovery occurs under basal conditions after the neutralization of venom components by antivenom. Thus, we aimed to investigate if BjV regulates gene expression of important hemostasis-related factors synthetized by the liver. On that account, Swiss mice were injected with saline or BjV (1.6 mg/kg b.w, s.c.), and after 3, 6 and 24 h blood samples and liver fragments were collected to analyze mRNA expression by real-time qPCR. Increased gene expression of fibrinogen chains, haptoglobin and STAT3 was observed during envenomation, particularly at 3 and 6 h. At 24h, mRNA levels of F10 were raised, while those of Serpinc1, Proc and Adamts13 were diminished. Surprisingly, F3 mRNA levels were steadily decreased at 3 h. Gene expression of Thpo, F7, F5 Tfpi, Mug1 was unaltered. mRNA levels of Vwf, P4hb, F8, F2, Plg, and Serpinf2 were minimally altered, but showed important associations with Nfkb1 gene expression. In conclusion, snakebite envenomation upregulates hepatic mRNA synthesis particularly of fibrinogen chains, and acute-phase markers. This response explains the fast recovery of fibrinogen levels after antivenom administration to patients bitten by B. jararaca snakes.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extente0008379pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPlos Neglected Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleLiver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in micept_BR
dc.typeArticlept_BR
dc.identifier.doi10.1371/journal.pntd.0008379pt_BR
dc.identifier.urlhttps://doi.org/10.1371/journal.pntd.0008379pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume14pt_BR
dc.identifier.citationissue6pt_BR
dc.relation.ispartofabbreviatedPLoS Negl Trop Dispt_BR
dc.identifier.citationabntv. 14, n. 6, e0008379, jun. 2020pt_BR
dc.identifier.citationvancouver2020 June;14(6):e0008379pt_BR
dc.contributor.butantanSachetto, Ana Teresa Azevedo|:Aluno|:Lab. Fisiopatologia|:PrimeiroAutorpt_BR
dc.contributor.butantanJensen, José Ricardo|:Pesquisador|:Lab. Imunogenéticapt_BR
dc.contributor.butantanSantoro, Marcelo Larami|:Pesquisador|:Lab. Fisiopatologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/25177-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/26015-8pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦312469/2018-7pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0001-6228-2988-
crisitem.author.orcid0000-0001-6364-3757-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.1371-journal.pntd.0008379.pdf
Description:
Size: 3.11 MB
Format: Adobe PDF
View/Open
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.