Differential coagulotoxicity of metalloprotease isoforms from Bothrops neuwiedi snake venom and consequent variations in antivenom efficacy

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dc.contributorLab. Imunopatologiapt_BR
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorSousa, Leijiane Figueira dept_BR
dc.contributor.authorBernardoni, Juliana Lechpt_BR
dc.contributor.authorZdenek, Christina N.pt_BR
dc.contributor.authorDobson, Jamespt_BR
dc.contributor.authorCoimbra, Franciscopt_BR
dc.contributor.authorGillett, Amberpt_BR
dc.contributor.authorLopes-Ferreira, Monicapt_BR
dc.contributor.authorMoura-da-Silva, Ana Mariapt_BR
dc.contributor.authorFry, Bryan G.pt_BR
dc.date.accessioned2020-08-28T14:07:37Z-
dc.date.available2020-08-28T14:07:37Z-
dc.date.issued2020pt_BR
dc.identifier.citationSousa LF, Bernardoni JL, Zdenek CN., Dobson J, Coimbra F, Gillett A, et al. Differential coagulotoxicity of metalloprotease isoforms from Bothrops neuwiedi snake venom and consequent variations in antivenom efficacy. Toxicol. Lett.. 2020 Oct;333:211-221. doi:10.1016/j.toxlet.2020.08.009.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3147-
dc.description.abstractBothrops (lance-head pit vipers) venoms are rich in weaponised metalloprotease enzymes (SVMP). These toxic enzymes are structurally diverse and functionally versatile. Potent coagulotoxicity is particularly important for prey capture (via stroke-induction) and relevant to human clinical cases (due to consumption of clotting factors including the critical depletion of fibrinogen). In this study, three distinct isoforms of P-III class SVMPs (IC, IIB and IIC), isolated from Bothrops neuwiedi venom, were evaluated for their differential capacities to affect hemostasis of prey and human plasma. Furthermore, we tested the relative antivenom neutralisation of effects upon human plasma. The toxic enzymes displayed differential procoagulant potency between plasma types, and clinically relevant antivenom efficacy variations were observed. Of particular importance was the confirmation the antivenom performed better against prothrombin activating toxins than Factor X activating toxins, which is likely due to the greater prevalence of the former in the immunising venoms used for antivenom production. This is clinically relevant as the enzymes displayed differential potency in this regard, with one (IC) in particular being extremely potent in activating Factor X and thus was correspondingly poorly neutralised. This study broadens the current understanding about the adaptive role of the SVMPs, as well as highlights how the functional diversity of SVMP isoforms can influence clinical outcomes.pt_BR
dc.description.sponsorshipAustralian Research Council (ARC)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent211-221pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxicology Letterspt_BR
dc.titleDifferential coagulotoxicity of metalloprotease isoforms from Bothrops neuwiedi snake venom and consequent variations in antivenom efficacypt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.toxlet.2020.08.009pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.toxlet.2020.08.009pt_BR
dc.contributor.externalUniversity of Queensland (UQ)pt_BR
dc.contributor.externalZoo and Wildlife Veterinary Consultancypt_BR
dc.identifier.citationvolume333pt_BR
dc.subject.keywordSVMPspt_BR
dc.subject.keywordHemorrhagept_BR
dc.subject.keywordCoagulotoxicitypt_BR
dc.subject.keywordEnvenomationpt_BR
dc.subject.keywordAdaptationpt_BR
dc.subject.keywordAntivenompt_BR
dc.subject.keywordVenompt_BR
dc.relation.ispartofabbreviatedToxicol Lettpt_BR
dc.identifier.citationabntv. 333, p. 211-221, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;333:211-221pt_BR
dc.contributor.butantanSousa, Leijiane Figueira de|:Aluno|:Lab. Imunopatologia|:PrimeiroAutorpt_BR
dc.contributor.butantanBernardoni, Juliana Lech|:Aluno|:Lab. Imunopatologiapt_BR
dc.contributor.butantanLopes-Ferreira, Monica Valdyrce dos Anjos|:Pesquisador:Docente permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanMoura-da-Silva, Ana Maria|:Pesquisador:Docente permanente PPGTOX|:Lab. Imunopatologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanAustralian Research Council (ARC)¦¦DP190100304pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/13124-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/15170-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2012/23018-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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item.languageiso639-1English-
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