Heterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cells

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dc.contributorLaboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorPessotti, Dayelle S.pt_BR
dc.contributor.authorAndrade-Silva, Déborapt_BR
dc.contributor.authorSerrano, Solange Maria de Toledopt_BR
dc.contributor.authorZelanis, Andrépt_BR
dc.date.accessioned2020-09-03T14:42:58Z-
dc.date.available2020-09-03T14:42:58Z-
dc.date.issued2020-
dc.identifier.citationPessotti DS., Andrade-Silva D, Serrano SMT, Zelanis A. Heterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cells. Biochim. Biophys. Acta Proteins. Proteom.. 2020 Dec;1868(12):140525. doi:10.1016/j.bbapap.2020.140525.pt_BR
dc.identifier.issn1878-1454-
dc.identifier.issn1570-9639-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3175-
dc.description.abstractThe signaling events triggered by soluble mediators released from both transformed and stromal cells shape the phenotype of tumoral cells and have significant implications in cancer development and progression. In this study we performed an in vitro heterotypic signaling assays by evaluating the proteome diversity of human dermal fibroblasts after stimulation with the conditioned media obtained from malignant melanoma cells. In addition, we also evaluated the changes in the proteome of melanoma cells after stimulation with their own conditioned media as well as with the conditioned medium from melanoma-stimulated fibroblasts. Our results revealed a clear rearrangement in the proteome of stromal and malignant cells upon crosstalk of soluble mediators. The main proteome signature of fibroblasts stimulated with melanoma conditioned medium was related to protein synthesis, which indicates that this process might be an early response of stromal cells. In addition, the conditioned medium derived from ‘primed’ stromal cells (melanoma-stimulated fibroblasts) was more effective in altering the functional phenotype (cell migration) of malignant cells than the conditioned medium from non-stimulated fibroblasts. Collectively, self- and cross-stimulation may play a key role in shaping the tumor microenvironment and enable tumoral cells to succeed in the process of melanoma progression and metastasis. Although the proteome landscape of cells participating in such a heterotypic signaling represents a snapshot of a highly dynamic state, understanding the diversity of proteins and enriched biological pathways resulting from stimulated cell states may allow for targeting specific cell regulatory motifs involved in melanoma progression and metastasis.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent140525pt_BR
dc.languageengpt_BR
dc.publisherElsevierpt_BR
dc.relation.ispartofBiochimica et biophysica Acta. Proteins and Proteomics.pt_BR
dc.titleHeterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cellspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.bbapap.2020.140525pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.bbapap.2020.140525pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.publisher.cityAmsterdampt_BR
dc.identifier.citationvolume1868pt_BR
dc.identifier.citationissue12pt_BR
dc.subject.keywordHeterotypic signalingpt_BR
dc.subject.keywordMelanomapt_BR
dc.subject.keywordFibroblastpt_BR
dc.subject.keywordCancer-associated fibroblastspt_BR
dc.subject.keywordPhenotypic plasticitypt_BR
dc.relation.ispartofabbreviatedBiochim. Biophys. Acta Proteins. Proteom.pt_BR
dc.identifier.citationabntv. 1868, n. 12, 140525, dez. 2020pt_BR
dc.identifier.citationvancouver2020 Dec;1868(12):140525pt_BR
dc.publisher.countryNetherlandspt_BR
dc.contributor.butantanAndrade-Silva, Débora|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.butantanSerrano, Solange Maria de Toledo|:Pesquisador:Docente permanente PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/06579-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/16935-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2019/07282-8pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
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item.grantfulltextembargo_29990101-
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