Geographic variation of individual venom profile of Crotalus durissus snakes

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dc.contributorLaboratório de Herpetologiapt_BR
dc.contributor.authorSilva-Júnior, Leandro Norberto dapt_BR
dc.contributor.authorAbreu, Lara De Souzapt_BR
dc.contributor.authorFabri, Carolinept_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorAguiar, Weslei da Silvapt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorSantos, Valdomiro Souza dospt_BR
dc.contributor.authorCosta, Isabella Alvespt_BR
dc.contributor.authorOliveira, Luis Vicente Francopt_BR
dc.contributor.authorSant'anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorTanaka-Azevedo, Anita Mitikopt_BR
dc.contributor.authorRodrigues, Leandro Nascimento da Silvapt_BR
dc.contributor.authorMorais-Zani, Karenpt_BR
dc.date.accessioned2020-09-15T19:43:32Z-
dc.date.available2020-09-15T19:43:32Z-
dc.date.issued2020pt_BR
dc.identifier.citationSilva-Júnior LN, Abreu LDS, Fabri C, Galizio NC, Aguiar WS, Silva CS, et al. Geographic variation of individual venom profile of Crotalus durissus snakes. J. Venom. Anim. Toxins Incl. Trop. Dis.. 2020 Aug;26:e20200016. doi:10.1590/1678-9199-jvatitd-2020-0016.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3191-
dc.description.abstractBackground: South American rattlesnakes are represented in Brazil by a single species, Crotalus durissus, which has public health importance due to the severity of its envenomation and to its wide geographical distribution. The species is subdivided into several subspecies, but the current classification is controversial. In Brazil, the venoms of C. d. terrificus and C. d. collilineatus are used for hyperimmunization of horses for antivenom production, even though the distinction of these two subspecies are mostly by their geographical distribution. In this context, we described a comparative compositional and functional characterization of individual C. d. collilineatus and C. d. terrificus venoms from three Brazilian states. Methods: We compared the compositional patterns of C. d. terrificus and C. d. collilineatus individual venoms by 1-DE and RP-HPLC. For functional analyzes, the enzymatic activities of PLA2, LAAO, and coagulant activity were evaluated. Finally, the immunorecognition of venom toxins by the crotalic antivenom produced at Butantan Institute was evaluated using Western blotting. Results: The protein profile of individual venoms from C. d. collilineatus and C. d. terrificus showed a comparable overall composition, despite some intraspecific variation, especially regarding crotamine and LAAO. Interestingly, HPLC analysis showed a geographic pattern concerning PLA2. In addition, a remarkable intraspecific variation was also observed in PLA2, LAAO and coagulant activities. The immunorecognition pattern of individual venoms from C. d. collilineatus and C. d. terrificus by crotalic antivenom produced at Butantan Institute was similar. Conclusions: The results highlighted the individual variability among the venoms of C. durissus ssp. specimens. Importantly, our data point to a geographical variation of C. durissus ssp. venom profile, regardless of the subspecies, as evidenced by PLA2 isoforms complexity, which may explain the increase in venom neurotoxicity from Northeastern through Southern Brazil reported for the species.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extente20200016pt_BR
dc.languageengpt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleGeographic variation of individual venom profile of Crotalus durissus snakespt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1590/1678-9199-jvatitd-2020-0016pt_BR
dc.identifier.urlhttp://dx.doi.org/10.1590/1678-9199-jvatitd-2020-0016pt_BR
dc.contributor.externalCentro Universitário de Anápolis (UniEVANGÉLICA)pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume26pt_BR
dc.subject.keywordIntraspecific venom variabilitypt_BR
dc.subject.keywordGeographic venom variabilitypt_BR
dc.subject.keywordCrotalus durissus collilineatuspt_BR
dc.subject.keywordCrotalus durissus terrificuspt_BR
dc.subject.keywordSnake venom composition and functionpt_BR
dc.relation.ispartofabbreviatedJ. Venom. Anim. Toxins Incl. Trop. Dis.pt_BR
dc.identifier.citationabntv. 26, e20200016, ago. 2020pt_BR
dc.identifier.citationvancouver2020 Aug;26:e20200016pt_BR
dc.contributor.butantanSilva-Júnior, Leandro Norberto da|:Aluno|:Laboratório de Herpetologia|:PrimeiroAutorpt_BR
dc.contributor.butantanAbreu, Lara De Souza|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanFabri, Caroline|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanAguiar, Weslei da Silva|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanSant'anna, Sávio Stefanini|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitiko|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanMorais-Zani, Karen|:Pesquisador|:Laboratório de Herpetologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/11108-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/09976-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/03839-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/26533-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/14724-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786-0pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦405399/2018-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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