Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?

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dc.contributorLab. Herpetologiapt_BR
dc.contributorLaboratório de Toxinologia Aplicadapt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorSimizo, Adrianapt_BR
dc.contributor.authorKitano, Eduardo S.pt_BR
dc.contributor.authorSant'anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorTashima, Alexandre Keijipt_BR
dc.identifier.citationSimizo A, Kitano ES., Sant'anna SS, Grego KF, Tanaka-Azevedo AM, Tashima AK. Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?. The journal of venomous animals and toxins. 2020 Oct;26:e20200055. doi:10.1590/1678-9199-jvatitd-2020-0055.pt_BR
dc.description.abstractBackground: Bothrops atrox is known to be the pit viper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including northern South America, the east of Andes and the Amazon basin. Possibly, due to its wide distribution and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: We conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that phospholipase A2 and bradykinin potentiating peptides were the most abundant peptide families in both genders, whereas disintegrin levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides as the Ba1a, which contains the SVMP BATXSVMPII1 catalytic site, were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in disintegrin levels in the venoms of females that may result in distinct pathophysiology of envenomation. Further research is required to explore the potential biological implications of this finding.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(FINEP) Financiadora de Estudos e Projetospt_BR
dc.description.sponsorship(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen accesspt_BR
dc.titleComparative gender peptidomics of Bothrops atrox venoms: are there differences between them?pt_BR
dc.rights.licenseCC BYpt_BR
dc.contributor.external(UNIFESP) Universidade Federal de São Paulopt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.subject.keywordBothrops atroxpt_BR
dc.subject.keywordSexual dimorphismpt_BR
dc.relation.ispartofabbreviatedJ Venom Anim Toxins Incl Trop Dispt_BR
dc.identifier.citationabntv. 26, e20200055, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;26:e20200055pt_BR
dc.contributor.butantanSant'anna, Sávio Stefanini|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanTashima, Alexandre Keiji|:Aluno|:Laboratório de Toxinologia Aplicada|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/20106-9pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/25786-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/03502-3pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦pt_BR
dc.sponsorship.butantan(CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior¦¦001pt_BR
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