Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?

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dc.contributorLaboratório de Herpetologiapt_BR
dc.contributorLaboratório Especial de Toxinologia Aplicada (LETA)pt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorSimizo, Adrianapt_BR
dc.contributor.authorKitano, Eduardo S.pt_BR
dc.contributor.authorSant'anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorTanaka-Azevedo, Anita Mitikopt_BR
dc.contributor.authorTashima, Alexandre Keijipt_BR
dc.identifier.citationSimizo A, Kitano ES., Sant'anna SS, Grego KF, Tanaka-Azevedo AM, Tashima AK. Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?. The journal of venomous animals and toxins. 2020 Oct;26:e20200055. doi:10.1590/1678-9199-jvatitd-2020-0055.pt_BR
dc.description.abstractBackground: Bothrops atrox is known to be the pit viper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including northern South America, the east of Andes and the Amazon basin. Possibly, due to its wide distribution and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: We conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that phospholipase A2 and bradykinin potentiating peptides were the most abundant peptide families in both genders, whereas disintegrin levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides as the Ba1a, which contains the SVMP BATXSVMPII1 catalytic site, were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in disintegrin levels in the venoms of females that may result in distinct pathophysiology of envenomation. Further research is required to explore the potential biological implications of this finding.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.publisherCenter for the Study of Venoms and Venomous Animals (CEVAP)pt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleComparative gender peptidomics of Bothrops atrox venomspt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.title.subare there differences between them?pt_BR
dc.subject.keywordBothrops atroxpt_BR
dc.subject.keywordSexual dimorphismpt_BR
dc.relation.ispartofabbreviatedThe journal of venomous animals and toxinspt_BR
dc.identifier.citationabntv. 26, e20200055, out. 2020-
dc.identifier.citationvancouver2020 Oct;26:e20200055-
dc.contributor.butantanSant'anna, Sávio Stefanini|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanTashima, Alexandre Keiji|:Aluno|:Laboratório Especial de Toxinologia Aplicada (LETA)|:Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)|:Autor de correspondênciapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/20106-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2019/03502-3pt_BR
dc.sponsorship.butantanFinanciadora de Estudos e Projetos (FINEP)¦¦pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
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