Prevalence of enteropathogens and virulence traits in Brazilian children with and without diarrhea

Full metadata record
DC FieldValueLanguage
dc.contributorLaboratório de Bacteriologiapt_BR
dc.contributor.authorMerino, Victor R.pt_BR
dc.contributor.authorNakano, Vivianept_BR
dc.contributor.authorDelannoy, Sabinept_BR
dc.contributor.authorFach, Patrickpt_BR
dc.contributor.authorAlberca, Gabriela G. F.pt_BR
dc.contributor.authorFarfan, Mauricio J.pt_BR
dc.contributor.authorPiazza, Roxane Maria Fontespt_BR
dc.contributor.authorAvila-Campos, Mario J.pt_BR
dc.date.accessioned2020-10-28T17:48:29Z-
dc.date.available2020-10-28T17:48:29Z-
dc.date.issued2020-
dc.identifier.citationMerino VR., Nakano V, Delannoy S, Fach P, Alberca GG.F., Farfan MJ., et al. Prevalence of enteropathogens and virulence traits in Brazilian children with and without diarrhea. Front. Cell. Infect. Microbiol.. 2020 Sept;10:549919. doi:10.3389/fcimb.2020.549919.pt_BR
dc.identifier.issn2235-2988-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3292-
dc.description.abstractThe use of molecular diagnostics for pathogen detection in epidemiological studies have allowed us to get a wider view of the pathogens associated with diarrhea, but the presence of enteropathogens in asymptomatic individuals has raised several challenges in understanding the etiology of diarrhea, and the use of these platforms in clinical diagnosis as well. To characterize the presence of the most relevant bacterial enteropathogens in diarrheal episodes, we evaluated here the prevalence of diarrheagenic E. coli pathotypes, Salmonella spp., and Yersinia enterocolitica in stool samples of children with and without diarrhea using real-time quantitative PCR (qPCR). We found that the presence of genetic markers associated with bacterial pathogens was significantly higher in stool samples from the diarrhea group compared to the control (P < 0.001). Bacterial loads in samples positive for eae and aggR markers were also determined. Compared to samples from asymptomatic children, a significantly higher number of copies of the eae gene were found in diarrhea samples. Also, the presence of genetic markers associated with STEC strains with clinical significance was evaluated in eae-positive samples by high-throughput real-time PCR. The data presented herein demonstrated that asymptomatic children of an urban area in Brazil might be enteropathogen reservoirs, especially for STEC.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipFondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT)pt_BR
dc.format.extent549919pt_BR
dc.languageengpt_BR
dc.publisherFrontiers Media SApt_BR
dc.relation.ispartofFrontiers in Cellular and Infection Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titlePrevalence of enteropathogens and virulence traits in Brazilian children with and without diarrheapt_BR
dc.typeArticlept_BR
dc.identifier.doihttps://repositorio.butantan.gov.br/handle/butantan/3292-
dc.identifier.doi10.3389/fcimb.2020.549919-
dc.identifier.urlhttps://doi.org/10.3389/fcimb.2020.549919-
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversité Paris-Estpt_BR
dc.contributor.externalUniversidad de Chilept_BR
dc.publisher.cityLausannept_BR
dc.identifier.citationvolume10-
dc.subject.keywordenteric pathogenspt_BR
dc.subject.keywordEscherichia coli pathotypespt_BR
dc.subject.keyworddiarrheal childrenpt_BR
dc.subject.keywordhealthy childrenpt_BR
dc.subject.keywordmolecular diagnosticspt_BR
dc.relation.ispartofabbreviatedFront. Cell. Infect. Microbiol.pt_BR
dc.identifier.citationabntv. 10, 549919. set. 2020-
dc.identifier.citationvancouver2020 Sept;10:549919-
dc.publisher.countrySwitzerlandpt_BR
dc.contributor.butantanPiazza, Roxane Maria Fontes|:Pesquisador|:Laboratório de Bacteriologia|:Autor de correspondênciapt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦158799/2012-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/17739-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/14821-7pt_BR
dc.sponsorship.butantanFondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT)¦¦1200994pt_BR
dc.identifier.bvsccBR78.1-
dc.identifier.bvsdbIBProd-
item.openairetypeArticle-
item.fulltextSem Texto completo-
item.grantfulltextnone-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos

Show simple item record

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.