Decellularized splenic matrix as a scaffold for spleen bioengineering

Full metadata record
DC FieldValueLanguage
dc.contributorLETA - Laboratório de Toxinologia Aplicadapt_BR
dc.contributorCentro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.contributor.authorZanardo, Tadeu Ériton Calimanpt_BR
dc.contributor.authorAmorim, Fernanda Gobbipt_BR
dc.contributor.authorTaufner, Gabriel Henriquept_BR
dc.contributor.authorPereira, Rayssa Helena Arrudapt_BR
dc.contributor.authorBaiense, Ian Manhonipt_BR
dc.contributor.authorDestefani, Afrânio Côgopt_BR
dc.contributor.authorIwai, Leo Keipt_BR
dc.contributor.authorMaranhão, Raul Cavalcantept_BR
dc.contributor.authorNogueira, Breno Valentimpt_BR
dc.date.accessioned2020-10-29T19:48:05Z-
dc.date.available2020-10-29T19:48:05Z-
dc.date.issued2020pt_BR
dc.identifier.citationZanardo TEC, Amorim FG, Taufner GH, Pereira RHA, Baiense IM, Destefani AC, et al. Decellularized splenic matrix as a scaffold for spleen bioengineering. Front. Bioeng. Biotechnol.. 2020 Oct;8:573461. doi:10.3389/fbioe.2020.573461.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3293-
dc.description.abstractThe spleen is considered a non-essential organ. However, its importance is increasingly clear, given the serious disorders caused by its absence or dysfunction, e.g., greater susceptibility to infections, thromboembolism and cancer. Surgical techniques to preserve the spleen and maintain splenic function have become increasingly common. However, the morbidity and mortality associated with its absence and dysfunction are still high. We used the decellularization technique to obtain a viable splenic scaffold for recellularization in vitro and propose the idea of bioengineered spleen transplantation to the host. We observed the maintenance of important structural components such as white pulp, marginal zone and red pulp, in addition to the network of vascular ducts. The decellularized scaffold presents minimal residual DNA and SDS, which are essential to prevent immunogenic responses and transplantation failure. Also, the main components of the splenic matrix were preserved after decellularization, with retention of approximately 72% in the matrisomal protein content. The scaffold we developed was partially recellularized with stromal cells from the spleen of neonatal rats, demonstrating adhesion, proliferation and viability of cells. Therefore, the splenic scaffold is very promising for use in studies on spleen reconstruction and transplantation, with the aim of complete recovery of splenic function.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Espírito Santo (FAPES)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent573461pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Bioengineering and Biotechnologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleDecellularized splenic matrix as a scaffold for spleen bioengineeringpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3389/fbioe.2020.573461pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fbioe.2020.573461pt_BR
dc.contributor.externalRede Nordeste de Biotecnologia (RENORBIO)pt_BR
dc.contributor.externalUniversidade Federal do Espírito Santo (UFES)pt_BR
dc.contributor.externalUniversidade Vila Velha (UVV)pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume8pt_BR
dc.subject.keywordsplenic scaffoldpt_BR
dc.subject.keyworddecellularizationpt_BR
dc.subject.keywordextracellular matrixpt_BR
dc.subject.keywordproteomic analysispt_BR
dc.subject.keywordrecellularizationpt_BR
dc.relation.ispartofabbreviatedFront Bioeng Biotechnolpt_BR
dc.identifier.citationabntv. 8, 573461, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;8:573461pt_BR
dc.contributor.butantanIwai, Leo Kei|:Pesquisador:Docente Colaborador PPGTOX|:Laboratório Especial de Toxinologia Aplicada (LETA):Centro de Toxinas, Resposta-imune e Sinalização Celular (CeTICS)pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦439439/2018-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Espírito Santo (FAPES)¦¦08/2019pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2013/07467-1pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/04000-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/17943-6pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦79522882/17pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1English-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-1571-7763-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.parentorg#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
Appears in Collections:Artigos de periódicos


Files in This Item:

10.3389fbioe.2020.573461.pdf
Description:
Size: 4.03 MB
Format: Adobe PDF
View/Open
Show simple item record

The access to the publications deposited in this repository respects the licenses from journals and publishers.