Virulent leptospira interrogans induce cytotoxic effects in human platelets in vitro through direct interactions

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dc.contributorLaboratório Especial de Desenvolvimento de Vacinas (LEDV)pt_BR
dc.contributor.authorVieira, Mônica Laruccipt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.date.accessioned2020-11-04T19:46:33Z-
dc.date.available2020-11-04T19:46:33Z-
dc.date.issued2020-
dc.identifier.citationVieira ML, Nascimento ALTO. Virulent leptospira interrogans induce cytotoxic effects in human platelets in vitro through direct interactions. Front. Microbiol.. 2020 Sept;11:572972. doi:10.3389/fmicb.2020.572972.pt_BR
dc.identifier.issn1664-302X-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3298-
dc.description.abstractLeptospirosis is a prevalent zoonotic disease, caused by bacteria of the genus Leptospira. Leptospirosis frequently leads to hemostatic disturbances, and the severe cases are marked by hemorrhages and low platelet number in circulation, which is associated with the patients’ poor outcomes. Nevertheless, Leptospira-platelet interactions remain poorly explored. In this study, we performed a series of in vitro experiments evaluating whether leptospires induce human platelet aggregation, activation, and morphological changes. Platelets were incubated with virulent L. interrogans and the platelet outcomes were assessed by aggregometry, flow cytometry, and scanning and transmission electron microscopy. Our results show that leptospires alone do not induce platelet aggregation and activation, and induce platelet cytotoxic effects instead, by clearly inducing platelet disruption and detachment. We show for the first time that virulent leptospires do interact directly with platelets, an event that could trigger pathophysiological effects during the infection. This study might serve as a basis for the development of novel treatments for the disease.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.extent572972pt_BR
dc.languageengpt_BR
dc.publisherFrontiers Research Foundationpt_BR
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleVirulent leptospira interrogans induce cytotoxic effects in human platelets in vitro through direct interactionspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.3389/fmicb.2020.572972-
dc.identifier.urlhttps://doi.org/10.3389/fmicb.2020.572972-
dc.contributor.externalUniversidade Federal de Minas Gerais (UFMG)pt_BR
dc.publisher.cityLausannept_BR
dc.identifier.citationvolume11-
dc.subject.keywordleptospirosispt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordblood plateletspt_BR
dc.subject.keywordplatelet aggregationpt_BR
dc.subject.keywordhemostasispt_BR
dc.subject.keywordhost-pathogen interactionspt_BR
dc.subject.keywordinfectious diseasespt_BR
dc.subject.keywordspirochetespt_BR
dc.relation.ispartofabbreviatedFront. Microbiol.pt_BR
dc.identifier.citationabntv. 11, 572972, set. 2020-
dc.identifier.citationvancouver2020 Sept;11:572972-
dc.publisher.countrySwitzerlandpt_BR
dc.contributor.butantanVieira, Mônica Larucci|:Aluno|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório Especial de Desenvolvimento de Vacinas (LEDV)pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/00236-5pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/50981-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/07054-2pt_BR
dc.identifier.bvsccBR78.1-
dc.identifier.bvsdbIBProd-
item.openairetypeArticle-
item.fulltextSem Texto completo-
item.grantfulltextnone-
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