In-depth transcriptome reveals the potential biotechnological application of Bothrops jararaca venom gland

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dc.contributorLab. Herpetologiapt_BR
dc.contributor.authorPereira, Leandro de Mattospt_BR
dc.contributor.authorMessias, Elisa Alvespt_BR
dc.contributor.authorSorroche, Bruna Pereirapt_BR
dc.contributor.authorOliveira, Angela das Nevespt_BR
dc.contributor.authorArantes, Lidia Maria Rebolho Batistapt_BR
dc.contributor.authorCarvalho, Ana Carolina dept_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorCarvalho, André Lopespt_BR
dc.contributor.authorMelendez, Matias Eliseopt_BR
dc.identifier.citationPereira LM, Messias EA, Sorroche BP, Oliveira AN, Arantes LMRB, Carvalho AC, et al. In-depth transcriptome reveals the potential biotechnological application of Bothrops jararaca venom gland. J. Venom. Anim. Toxins Incl. Trop. Dis.. 2020 Oct;26:e20190058. doi:10.1590/1678-9199-jvatitd-2019-0058.pt_BR
dc.description.abstractBackground: Lack of complete genomic data of Bothrops jararaca impedes molecular biology research focusing on biotechnological applications of venom gland components. Identification of full-length coding regions of genes is crucial for the correct molecular cloning design. Methods: RNA was extracted from the venom gland of one adult female specimen of Bothrops jararaca. Deep sequencing of the mRNA library was performed using Illumina NextSeq 500 platform. De novo assembly of B. jararaca transcriptome was done using Trinity. Annotation was performed using Blast2GO. All predicted proteins after clustering step were blasted against non-redundant protein database of NCBI using BLASTP. Metabolic pathways present in the transcriptome were annotated using the KAAS-KEGG Automatic Annotation Server. Toxins were identified in the B. jararaca predicted proteome using BLASTP against all protein sequences obtained from Animal Toxin Annotation Project from Uniprot KB/Swiss-Pro database. Figures and data visualization were performed using ggplot2 package in R language environment. Results: We described the in-depth transcriptome analysis of B. jararaca venom gland, in which 76,765 de novo assembled isoforms, 96,044 transcribed genes and 41,196 unique proteins were identified. The most abundant transcript was the zinc metalloproteinase-disintegrin-like jararhagin. Moreover, we identified 78 distinct functional classes of proteins, including toxins, inhibitors and tumor suppressors. Other venom proteins identified were the hemolytic lethal factors stonustoxin and verrucotoxin. Conclusion: It is believed that the application of deep sequencing to the analysis of snake venom transcriptomes may represent invaluable insight on their biotechnological potential focusing on candidate molecules.pt_BR
dc.description.sponsorshipPrograma de Incentivo ao Apoio à Pesquisa (PAIP)pt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsOpen Accesspt_BR
dc.titleIn-depth transcriptome reveals the potential biotechnological application of Bothrops jararaca venom glandpt_BR
dc.contributor.externalHospital de Câncer de Barretopt_BR
dc.contributor.externalUniversidade Federal do Rio de Janeiro (UFRJ)pt_BR
dc.contributor.externalInstituto de Pesquisa Pelé Pequeno Príncipept_BR
dc.subject.keywordBothrops jararacapt_BR
dc.subject.keywordVenom glandpt_BR
dc.subject.keywordBiotechnological applicationpt_BR
dc.relation.ispartofabbreviatedJ Venom Anim Toxins Incl Trop Dispt_BR
dc.identifier.citationabntv. 26, e20190058, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;26:e20190058pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Lab. Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Lab. Herpetologiapt_BR
dc.sponsorship.butantanPrograma de Incentivo ao Apoio à Pesquisa (PAIP)¦¦pt_BR
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