Immunoprotective activity induced by leptospiral outer membrane proteins in hamster model of acute leptospirosis

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dc.contributorLab. Desenvolvimento de Vacinaspt_BR
dc.contributor.authorTeixeira, Aline Rodrigues Florênciopt_BR
dc.contributor.authorPereira, Maria Fernanda Cavenaguept_BR
dc.contributor.authorKochi, Leandro Toshiopt_BR
dc.contributor.authorFernandes, Luis Guilherme Vírgíliopt_BR
dc.contributor.authorSouza, Gisele Opt_BR
dc.contributor.authorSouza Filho, Antonio Francisco dept_BR
dc.contributor.authorVasconcellos, Silvio A.pt_BR
dc.contributor.authorHeinemann, Marcos Bryanpt_BR
dc.contributor.authorNascimento, Ana Lúcia Tabet Oller dopt_BR
dc.date.accessioned2020-11-27T19:11:11Z-
dc.date.available2020-11-27T19:11:11Z-
dc.date.issued2020pt_BR
dc.identifier.citationTeixeira ARF, Pereira MFC, Kochi LT, Fernandes LGV, Souza GO, Souza Filho AF, et al. Immunoprotective activity induced by leptospiral outer membrane proteins in hamster model of acute leptospirosis. Front. Immunol.. 2020 Oct;10:3389. doi:10.3389/fimmu.2020.568694.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3313-
dc.description.abstractLeptospirosis is a zoonotic disease of worldwide distribution, affecting both humans and animals. The development of an effective vaccine against leptospirosis has long been pursued but without success. Humans are contaminated after direct contact with the urine of infected animals or indirectly by contaminated water or soil. The vaccines available consist of inactivated whole-bacterial cells, and the active immunoprotective antigen is the lipopolysaccharide moiety, which is also the basis for serovar classification. However, these vaccines are short-lasting, and protection is only against serovars contained in the preparation. The search for prevalent antigens, present in pathogenic species of Leptospira, represents the most cost-effective strategy for prevention of leptospirosis. Thus, the identification of these antigens is a priority. In this study, we examined the immunoprotective effect of eight leptospiral recombinant proteins using hamster as the challenge model. Animals received subcutaneously two doses of vaccine containing 50 μg of each recombinant protein adsorbed on alum adjuvant. Two weeks after the booster, animals were challenged with virulent leptospires and monitored for 21 days. All proteins were able to induce a specific immune response, although significant protective effects on survival rate were observed only for the proteins Lsa14, rLIC13259, and rLIC11711. Of these, only rLIC13259 and rLIC11711 were found to be highly prospective in promoting renal clearance. The sterilizing potential of both proteins will be further investigated to elucidate the immunoprotective mechanisms involved in leptospirosis control. These are the first proteins involved with human complement components with the capacity to protect against virulent challenge and to eliminate the bacteria from the host.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.extent568694pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofFrontiers in Immunologypt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleImmunoprotective activity induced by leptospiral outer membrane proteins in hamster model of acute leptospirosispt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3389/fimmu.2020.568694pt_BR
dc.identifier.urlhttps://doi.org/10.3389/fimmu.2020.568694pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume11pt_BR
dc.subject.keywordLeptospirapt_BR
dc.subject.keywordleptospirosispt_BR
dc.subject.keywordvaccinept_BR
dc.subject.keywordrecombinant proteinspt_BR
dc.subject.keywordimmunoprotectionpt_BR
dc.subject.keywordimmunogenicitypt_BR
dc.relation.ispartofabbreviatedFront Immunolpt_BR
dc.identifier.citationabntv. 10, 568694, out. 2020pt_BR
dc.identifier.citationvancouver2020 Oct;10:568694pt_BR
dc.contributor.butantanTeixeira, Aline Rodrigues Florêncio|:Aluno|:Laboratório de Desenvolvimento de Vacinas|:PrimeiroAutorpt_BR
dc.contributor.butantanPereira, Maria Fernanda Cavenague|:Aluno|:Laboratório de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanKochi, Leandro Toshio|:Aluno|:Laboratório de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanFernandes, Luis Guilherme Vírgílio|:Aluno|:Laboratório de Desenvolvimento de Vacinaspt_BR
dc.contributor.butantanNascimento, Ana Lúcia Tabet Oller do|:Pesquisador|:Laboratório de Desenvolvimento de Vacinas|:Autor de correspondênciapt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2014/50981-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/08131-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2018/09652-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/06731-8pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2016/11541-0pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦301229/2017-1pt_BR
dc.sponsorship.butantanFundação Butantan¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
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