SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network

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dc.contributorLaboratório de Virologiapt_BR
dc.contributor.authorAraujo, Danielle Bastospt_BR
dc.contributor.authorMachado, Rafael Rahal Guaragnapt_BR
dc.contributor.authorAmgarten, Deyvid Emanuelpt_BR
dc.contributor.authorMalta, Fernanda de Mellopt_BR
dc.contributor.authorAraujo, Gabriel Guarany dept_BR
dc.contributor.authorMonteiro, Cairo Oliveirapt_BR
dc.contributor.authorCandido, Erika Donizettipt_BR
dc.contributor.authorSoares, Camila Pereirapt_BR
dc.contributor.authorMenezes, Fernando Gatti dept_BR
dc.contributor.authorPires, Ana Carolina Cornachionipt_BR
dc.contributor.authorSantana, Rúbia Anita Ferrazpt_BR
dc.contributor.authorViana, Amanda de Oliveirapt_BR
dc.contributor.authorDorlass, Erickpt_BR
dc.contributor.authorThomazelli, Lucianopt_BR
dc.contributor.authorFerreira, Luis Carlos de Sousapt_BR
dc.contributor.authorBotosso, Viviane Fongaropt_BR
dc.contributor.authorCarvalho, Cristiane Rodrigues Guzzopt_BR
dc.contributor.authorOliveira, Danielle Bruna Leal dept_BR
dc.contributor.authorPinho, João Renato Rebellopt_BR
dc.contributor.authorDurigon, Edison Luizpt_BR
dc.date.accessioned2020-12-04T19:29:17Z-
dc.date.available2020-12-04T19:29:17Z-
dc.date.issued2020-
dc.identifier.citationAraujo DB, Machado RRG, Amgarten DE, Malta FM, Araujo GG, Monteiro CO, et al. SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. Mem. Inst. Oswaldo Cruz. 2020;115:e200342. doi:10.1590/0074-02760200342.pt_BR
dc.identifier.issn0074-0276-
dc.identifier.issn1678-8060-
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3347-
dc.description.abstractBACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extente200342pt_BR
dc.languageengpt_BR
dc.publisherInstituto Oswaldo Cruzpt_BR
dc.relation.ispartofMemórias do Instituto Oswaldo Cruzpt_BR
dc.rightsOpen Accesspt_BR
dc.titleSARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task networkpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1590/0074-02760200342pt_BR
dc.identifier.urlhttps://doi.org/10.1590/0074-02760200342pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalHospital Israelita Albert Einsteinpt_BR
dc.publisher.cityRio de Janeiropt_BR
dc.identifier.citationvolume115pt_BR
dc.subject.keywordSARS-CoV-2pt_BR
dc.subject.keywordCOVID-19pt_BR
dc.subject.keywordvirus isolationpt_BR
dc.subject.keywordcell culturept_BR
dc.subject.keywordvirus networkpt_BR
dc.relation.ispartofabbreviatedMem. Inst. Oswaldo Cruzpt_BR
dc.identifier.citationabntv. 115, e200342, 2020pt_BR
dc.identifier.citationvancouver2020;115:e200342pt_BR
dc.publisher.countryBrazilpt_BR
dc.contributor.butantanBotosso, Viviane Fongaro|:Pesquisador|:Laboratório de Virologiapt_BR
dc.contributor.butantanOliveira, Danielle Bruna Leal de|:Aluno|:Laboratório de Virologiapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/24769-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/21076-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2020/04680-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/23680-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/25123-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/20045-7pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2020/06409-1pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦88887.131387/2016-00pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦440409/2016-0pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
item.openairetypeArticle-
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item.grantfulltextembargo_29990101-
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