Método para obter lipídeo a monofosforilado da bordetella pertussis como um subproduto da produção de vacina celular contra coqueluche

Abstract

Method for obtaining monophosphorylated lipid from bordetella pertussis as a by-product of cellular pertussis vaccine production, more specifically, a method of purifying bpmpla from the processing of a generally rejected or filtered alcoholic extract of inactivated collection of bordetella pertus vaccine. sis (by-product of the main vaccine production, bpmpla purification method which comprises processing a generally rejected alcoholic extract from the inactivated collection of bordetella pertussis, submitting this final extract to an affinity column and sending the eluted detergent fraction to acid hydrolysis followed by neutralization, lyophilization and resuspension with water for injection plus a mild detergent; the new bpmpla is used in immunostimulating applications, more specifically, as a vaccine adjuvant and, even more specifically, as an influenza vaccine adjuvant, with promising results.