A Representative GIIA phospholipase A2 activates preadipocytes to produce inflammatory mediators implicated in obesity development

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dc.contributorLab. Farmacologiapt_BR
dc.contributor.authorLeiguez Junior, Elbiopt_BR
dc.contributor.authorMotta, Priscila Signorpt_BR
dc.contributor.authorMarques, Rodrigo Maiapt_BR
dc.contributor.authorLomonte, Brunopt_BR
dc.contributor.authorSampaio, Suely Vilelapt_BR
dc.contributor.authorTeixeira, Catarina de Fátima Pereirapt_BR
dc.date.accessioned2020-12-16T18:08:09Z-
dc.date.available2020-12-16T18:08:09Z-
dc.date.issued2020pt_BR
dc.identifier.citationLeiguez Junior E, Motta PS, Marques RM, Lomonte B, Sampaio SV, Teixeira C. A Representative GIIA phospholipase A2 activates preadipocytes to produce inflammatory mediators implicated in obesity development. Biomolecules. 2020 Nov;10(12):1593. doi:10.3390/biom10121593.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3375-
dc.description.abstractAdipose tissue secretes proinflammatory mediators which promote systemic and adipose tissue inflammation seen in obesity. Group IIA (GIIA)-secreted phospholipase A2 (sPLA2) enzymes are found to be elevated in plasma and adipose tissue from obese patients and are active during inflammation, generating proinflammatory mediators, including prostaglandin E2 (PGE2). PGE2 exerts anti-lipolytic actions and increases triacylglycerol levels in adipose tissue. However, the inflammatory actions of GIIA sPLA2s in adipose tissue cells and mechanisms leading to increased PGE2 levels in these cells are unclear. This study investigates the ability of a representative GIIA sPLA2, MT-III, to activate proinflammatory responses in preadipocytes, focusing on the biosynthesis of prostaglandins, adipocytokines and mechanisms involved in these effects. Our results showed that MT-III induced biosynthesis of PGE2, PGI2, MCP-1, IL-6 and gene expression of leptin and adiponectin in preadipocytes. The MT-III-induced PGE2 biosynthesis was dependent on cytosolic PLA2 (cPLA2)-α, cyclooxygenases (COX)-1 and COX-2 pathways and regulated by a positive loop via the EP4 receptor. Moreover, MT-III upregulated COX-2 and microsomal prostaglandin synthase (mPGES)-1 protein expression. MCP-1 biosynthesis induced by MT-III was dependent on the EP4 receptor, while IL-6 biosynthesis was dependent on EP3 receptor engagement by PGE2. These data highlight preadipocytes as targets for GIIA sPLA2s and provide insight into the roles played by this group of sPLA2s in obesity.pt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent1593pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofBiomoleculespt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleA Representative GIIA phospholipase A2 activates preadipocytes to produce inflammatory mediators implicated in obesity developmentpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/biom10121593pt_BR
dc.identifier.urlhttps://doi.org/10.3390/biom10121593pt_BR
dc.contributor.external(UCR) Universidad de Costa Ricapt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume10pt_BR
dc.identifier.citationissue12pt_BR
dc.subject.keywordphospholipase A2pt_BR
dc.subject.keywordpreadipocytespt_BR
dc.subject.keywordprostaglandinspt_BR
dc.subject.keywordadipokinespt_BR
dc.subject.keywordcytokinespt_BR
dc.subject.keywordEP receptorspt_BR
dc.relation.ispartofabbreviatedBiomoleculespt_BR
dc.identifier.citationabntv. 10, n. 12, 1593, nov. 2020pt_BR
dc.identifier.citationvancouver2020 Nov;10(12):1593pt_BR
dc.contributor.butantanLeiguez, Elbio|:Aluno|:Lab. Farmacologia|:PrimeiroAutor:Autor de correspondênciapt_BR
dc.contributor.butantanMotta, Priscila Signor|:Aluno|:Lab. Farmacologiapt_BR
dc.contributor.butantanMarques, Rodrigo Maia|:Aluno|:Lab. Farmacologiapt_BR
dc.contributor.butantanTeixeira, Catarina de Fátima Pereira|:Pesquisador:Docente permanente PPGTOX|:Lab. Farmacologia|:Autor de correspondências 2011/23236-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2011/23236-4pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2015/24701-3pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2017/197339pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.grantfulltextopen-
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
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