Silent mutations in ribosomal protein genes are associated with high-risk clones of carbapenem-resistant Acinetobacter baumannii prevalent in Brazil

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dc.contributorLab. Bacteriologiapt_BR
dc.contributor.authorBarcellos, Thays Almeida Franco dept_BR
dc.contributor.authorBueno, Mariana Sardinhapt_BR
dc.contributor.authorCunha, Marcos Paulo Vieirapt_BR
dc.contributor.authorNagamori, Filipe Onishipt_BR
dc.contributor.authorCarvalho, Eneaspt_BR
dc.contributor.authorTakagi, Elizabeth Harummyypt_BR
dc.contributor.authorMoreno, Luisa Zanollipt_BR
dc.contributor.authorMoreno, Andrea Mickept_BR
dc.contributor.authorChimara, Ericapt_BR
dc.contributor.authorTiba-Casas, Monique Ribeiropt_BR
dc.contributor.authorCamargo, Carlos Henriquept_BR
dc.date.accessioned2021-01-05T18:17:09Z-
dc.date.available2021-01-05T18:17:09Z-
dc.date.issued2021pt_BR
dc.identifier.citationBarcellos TAF, Bueno MS, Cunha MPV, Nagamori FO, Carvalho E, Takagi EH, et al. Silent mutations in ribosomal protein genes are associated with high-risk clones of carbapenem-resistant Acinetobacter baumannii prevalent in Brazil. Infect. Genet. Evol.. 2021 Mar;88:104686. doi:10.1016/j.meegid.2020.104686.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3428-
dc.description.abstractObjectives: To analyze the relationship of ribosomal protein mutations and clonality of high-risk clones Acinetobacter baumannii. Methods: Seventy-nine carbapenem-resistant A. baumannii were subjected to whole-genome sequencing (Illumina NextSeq), and codifying sequences of ribosomal proteins were extracted and screened for mutations. MALDI-TOF MS analysis (Bruker Biotyper) and Spectra data from MALDI-TOF was employed to generate a dendrogram based on principal component analysis (PCA) data. Clones were identified by Multilocus sequencing typing (MLST) based on WGS. Results: Ribosomal RNA protein sequences extracted from the genomes identified mutations that were associated with clonal complexes, but most of them were silent. PCA did not cluster the isolates according to their clonality identified by MLST. Conclusions: By comparing the nucleotide and amino acid sequences of diversified A. baumannii, and Bruker Biotyper profiles, we showed that silent mutations in ribosomal RNA nucleotides are associated with clonal complexes, but since most of the mutations were silent, MALDI-TOF MS raw data was not a useful tool for typing the high-risk clones of this species.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent104686pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofInfection, Genetics and Evolutionpt_BR
dc.titleSilent mutations in ribosomal protein genes are associated with high-risk clones of carbapenem-resistant Acinetobacter baumannii prevalent in Brazilpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.meegid.2020.104686pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.meegid.2020.104686pt_BR
dc.contributor.externalInstituto Adolfo Lutz (IAL)pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.identifier.citationvolume88pt_BR
dc.subject.keywordMLSTpt_BR
dc.subject.keywordMALDI-TOFpt_BR
dc.subject.keywordBrukerpt_BR
dc.subject.keywordHigh-risk clonespt_BR
dc.subject.keywordMass spectrometrypt_BR
dc.subject.keywordWGSpt_BR
dc.relation.ispartofabbreviatedInfect Genet Evolpt_BR
dc.identifier.citationabntv. 8, 104686, mar. 2021pt_BR
dc.identifier.citationvancouver2021 Mar;8:104686pt_BR
dc.contributor.butantanCarvalho, Eneas|:Pesquisador|:Lab. Bacteriologiapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16988-6pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.grantfulltextembargo_29990101-
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