Cell separation of Haemophilus influenzae type b through tangential microfiltration

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dc.contributorLab. Desenvolvimento de Processospt_BR
dc.contributor.authorBraga, Luciano Gamapt_BR
dc.contributor.authorCabrera-Crespo, Joaquinpt_BR
dc.contributor.authorTakagi, Mickiept_BR
dc.date.accessioned2021-01-13T20:20:11Z-
dc.date.available2021-01-13T20:20:11Z-
dc.date.issued2021pt_BR
dc.identifier.citationBraga LG, Cabrera-Crespo J, Takagi M. Cell separation of Haemophilus influenzae type b through tangential microfiltration. Sep. Purif. Technol.. 2021 Feb;257:117965. doi:10.1016/j.seppur.2020.117965.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3442-
dc.description.abstractCentrifugation techniques are frequently used to separate bacteria from the culture broth in pharmaceutical industries. Alternatively, cell separation can be performed through tangential microfiltration systems; an arguably cost-effective alternative to centrifugation. Therefore, replacement of centrifugation steps with microfiltration represents an attractive option in order to decrease production costs. An example of such use can be found in the production of the vaccine against Haemophilus influenzae type b (Hib), which relies on a centrifugation step to separate the pathogenic bacteria from the antigen-rich (exopolysaccharide) released into culture broth. The substitution of the centrifugation operation with tangential microfiltration may decrease production costs and increase vaccine availability in low-income regions. Hence, we studied the impact of diverse microfiltration systems at different production scales in the separation of Hib from its culture broth. The recovery of the exopolysaccharide - polyribosylribitol phosphate (PRP), the antigen employed in the vaccine, produced by Hib and present in the culture broth was used as a read-out for process efficiency. In sum, the use of Hydrophilic Polyvinylidene Fluoride (PVDF) membranes resulted in the highest recovery value among the tested materials; moreover, the transmembrane pressure was a paramount factor determining the recovery level. We concluded that Hib cell separation through tangential microfiltration systems represents a feasible alternative to centrifugation.pt_BR
dc.description.sponsorshipBanco Nacional de Desenvolvimento Econômico e Social (BNDES)pt_BR
dc.description.sponsorshipFundação Butantanpt_BR
dc.format.extent117965pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofSeparation and Purification Technologypt_BR
dc.titleCell separation of Haemophilus influenzae type b through tangential microfiltrationpt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.seppur.2020.117965pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.seppur.2020.117965pt_BR
dc.identifier.citationvolume257pt_BR
dc.subject.keywordCell separationpt_BR
dc.subject.keywordExopolysaccharide PRPpt_BR
dc.subject.keywordIndustrial scalept_BR
dc.subject.keywordPilot-scalept_BR
dc.subject.keywordTangential microfiltrationpt_BR
dc.subject.keywordVaccine productionpt_BR
dc.relation.ispartofabbreviatedSep Purif Technolpt_BR
dc.identifier.citationabntv. 257, 117965, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Feb;257:117965pt_BR
dc.contributor.butantanBraga, Luciano Gama|:Estagiário|:Lab. Desenvolvimento de Processos|:PrimeiroAutorpt_BR
dc.contributor.butantanCabrera-Crespo, Joaquin|:Pesquisador|:Lab. Desenvolvimento de Processospt_BR
dc.contributor.butantanTakagi, Mickie|:Pesquisador|:Lab. Desenvolvimento de Processos|:Autor de correspondênciapt_BR
dc.sponsorship.butantanBanco Nacional de Desenvolvimento Econômico e Social (BNDES)¦¦11.2.0322.1/2012pt_BR
dc.sponsorship.butantanFundação Butantan¦¦pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.openairetypeArticle-
item.fulltextCom Texto completo-
item.grantfulltextembargo_29990101-
item.languageiso639-1English-
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crisitem.author.dept#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.author.orcid0000-0002-1457-6786-
crisitem.author.orcid0000-0002-0978-4070-
crisitem.journal.journalissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
crisitem.journal.journaleissn#PLACEHOLDER_PARENT_METADATA_VALUE#-
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