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Biocompatible lipid polymer cationic nanoparticles for antigen presentation

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dc.contributorLab. Imunopatologiapt_BR
dc.contributor.authorPérez-Betancourt, Yunyspt_BR
dc.contributor.authorTávora, Bianca de Carvalho Lins Fernandespt_BR
dc.contributor.authorFaquim Mauro, Eliana Limapt_BR
dc.contributor.authorAna Maria Carmona-Ribeiropt_BR
dc.date.accessioned2021-01-15T13:54:27Z-
dc.date.available2021-01-15T13:54:27Z-
dc.date.issued2021pt_BR
dc.identifier.citationPérez-Betancourt Y, Távora BCLF, Faquim Mauro EL, AMC-R. Biocompatible lipid polymer cationic nanoparticles for antigen presentation. Polymers.. 2021 Jan;13:185. doi:10.3390/polym13020185.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3445-
dc.description.abstractBiocompatible lipid polymer nanoparticles (NPs) previously used as antimicrobial agents are explored here as immuno-adjuvants. Poly (methyl methacrylate) (PMMA)/dioctadecyldimethylammonium bromide (DODAB)/poly (diallyldimethylammonium chloride) (PDDA) nanoparticles (NPs) were prepared by emulsion polymerization of methyl methacrylate (MMA) in the presence of DODAB and PDDA, with azobisisobutyronitrile (AIBN) as the initiator. NPs characterization after dialysis by dynamic light-scattering yielded 225 ± 2 nm hydrodynamic diameter (Dz), 73 ± 1 mV zeta-potential (ζ), and 0.10 ± 0.01 polydispersity (P). Ovalbumin (OVA) adsorption reduced ζ to 45 ± 2 mV. Balb/c mice immunized with NPs/OVA produced enhanced OVA-specific IgG1 and IgG2a, exhibited moderate delayed type hypersensitivity reaction, and enhanced cytokines production (IL-4, IL-10, IL-2, IFN-γ) by cultured spleen cells. There was no cytotoxicity against cultured macrophages and fibroblasts. Advantages of the PMMA/DODAB/PDDA NPs were high biocompatibility, zeta-potential, colloidal stability, and antigen adsorption. Both humoral and cellular antigen-specific immune responses were obtained.pt_BR
dc.description.sponsorship(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorship(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.extent185pt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofPolymerspt_BR
dc.rightsOpen accesspt_BR
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_BR
dc.titleBiocompatible lipid polymer cationic nanoparticles for antigen presentationpt_BR
dc.typeArticlept_BR
dc.rights.licenseCC BYpt_BR
dc.identifier.doi10.3390/polym13020185pt_BR
dc.identifier.urlhttps://doi.org/10.3390/polym13020185pt_BR
dc.contributor.external(USP) Universidade de São Paulopt_BR
dc.identifier.citationvolume13pt_BR
dc.identifier.citationissue2pt_BR
dc.subject.keywordhumoral and cellular immune responsespt_BR
dc.subject.keywordcationic lipidpt_BR
dc.subject.keywordcationic polymerpt_BR
dc.subject.keywordovalbuminpt_BR
dc.subject.keywordhybrid nanoparticlespt_BR
dc.subject.keywordprotein deliverypt_BR
dc.subject.keywordvaccine adjuvantspt_BR
dc.subject.keywordcationic nanoparticles toxicitypt_BR
dc.relation.ispartofabbreviatedPolymerspt_BR
dc.identifier.citationabntv. 13, n. 2, 185, jan, 2021.pt_BR
dc.identifier.citationvancouver2021 Jan;13(2):185pt_BR
dc.contributor.butantanTávora, Bianca de Carvalho Lins Fernandes|:Técnico|:Lab. Imunopatologiapt_BR
dc.contributor.butantanFaquim Mauro, Eliana Lima|:Pesquisador|:Lab. Imunopatologiapt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦302758/2019-4pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦140091/2019-0pt_BR
dc.sponsorship.butantan(FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo¦¦2019/17685-2pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦302352/2014-7pt_BR
dc.sponsorship.butantan(CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico¦¦312096/2018-6pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.fulltextCom Texto completo-
item.openairetypeArticle-
item.languageiso639-1English-
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