Snake venom color and L-amino acid oxidase: an evidence of long-term captive Crotalus durissus terrificus venom plasticity

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dc.contributorLaboratório de Herpetologiapt_BR
dc.contributor.authorLima, Eduardo Oliveira Venâncio dept_BR
dc.contributor.authorTasima, Lidia Jorgept_BR
dc.contributor.authorHatakeyama, Daniela Mikipt_BR
dc.contributor.authorSilva, Caroline Serinopt_BR
dc.contributor.authorFabri, Carolinept_BR
dc.contributor.authorGalizio, Nathália da Costapt_BR
dc.contributor.authorChiarelli, Tassiapt_BR
dc.contributor.authorNishiduka, Erika Sayuript_BR
dc.contributor.authorRocha, Marisa Maria Teixeira dapt_BR
dc.contributor.authorSant'Anna, Sávio Stefaninipt_BR
dc.contributor.authorGrego, Kathleen Fernandespt_BR
dc.contributor.authorTashima, Alexandre Keijipt_BR
dc.contributor.authorTanaka-Azevedo, Anita Miticopt_BR
dc.contributor.authorMorais-Zani, Karen dept_BR
dc.date.accessioned2021-02-02T18:37:05Z-
dc.date.available2021-02-02T18:37:05Z-
dc.date.issued2021pt_BR
dc.identifier.citationLima EOV, Tasima LJ, Hatakeyama DM, Silva CS, Fabri C, Galizio NC, et al. Snake venom color and L-amino acid oxidase: an evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon X. 2021 Jan;193:73-83. doi:10.1016/j.toxicon.2021.01.006.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3504-
dc.description.abstractThe venom color variation of Crotalus durissus terrificus (Cdt) is attributed to the presence of the toxin L-amino acid oxidase (LAAO). During the venom milking routine of Instituto Butantan, we have noticed that most venoms of captive Cdt specimens show a yellowish color, while most venoms of wild specimens are white. Here we describe a comparative analysis of long-term captive (LTC) and recently wild-caught (RWC) Cdt, focusing on LAAO variation. For the identification of LAAO in individual venoms, four different approaches were employed: evaluation of the enzymatic activity, SDS-PAGE, Western blotting, and ELISA. In addition, mass spectrometry analysis was performed using pooled samples. Although some variation among these methodologies was observed, it was possible to notice that LAAOs presence were significantly higher in the venom of LTC individuals. LAAO was identified in 60-80% LTC specimens and in only 10-12% of RWC specimens. Furthermore, this enzyme accounts for 5.6% of total venom proteins of LTC Cdt pooled venom, while it corresponds to only 0.7% of RWC Cdt pooled venom. These findings strongly suggest that captive maintenance increases the expression of LAAO in Cdt venom.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.format.extent73-83pt_BR
dc.languageengpt_BR
dc.language.isoEnglishpt_BR
dc.relation.ispartofToxicon: Xpt_BR
dc.rightsOpen Accesspt_BR
dc.titleSnake venom color and L-amino acid oxidase: an evidence of long-term captive Crotalus durissus terrificus venom plasticitypt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.toxicon.2021.01.006pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.toxicon.2021.01.006pt_BR
dc.contributor.externalUniversidade de São Paulo (USP)pt_BR
dc.contributor.externalUniversidade Federal de São Paulo (UNIFESP)pt_BR
dc.identifier.citationvolume193pt_BR
dc.relation.ispartofabbreviatedToxicon Xpt_BR
dc.identifier.citationabntv. 193, p. 73-83, jan. 2021pt_BR
dc.identifier.citationvancouver2021 Jan;193:73-83pt_BR
dc.contributor.butantanLima, Eduardo Oliveira Venâncio de|:Aluno|:Laboratório de Herpetologia|:PrimeiroAutorpt_BR
dc.contributor.butantanTasima, Lidia Jorge|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanHatakeyama, Daniela Miki|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanSilva, Caroline Serino|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanFabri, Caroline|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGalizio, Nathália da Costa|:Aluno|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanRocha, Marisa Maria Teixeira da|:Técnico|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanSant'Anna, Sávio Stefanini|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanGrego, Kathleen Fernandes|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanTashima, Alexandre Keiji|:Aluno|:pt_BR
dc.contributor.butantanTanaka-Azevedo, Anita Mitico|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.contributor.butantanKaren de Morais-Zani|:Pesquisador|:Laboratório de Herpetologiapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2014/11108-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/09976-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2016/03839-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/01890-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/16908-2pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/26533-3pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/20106-9pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2017/21052-0pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/14724-4pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)¦¦2018/25786-0pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦405399/2018-9pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
dc.description.dbindexedYespt_BR
item.languageiso639-1English-
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