Differential expression of Acanthamoeba castellanii proteins during amoebic keratitis in rats

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dc.contributorLaboratório de Bioquímica e Biofísicapt_BR
dc.contributor.authorCarvalho-Silva, Ana Carolinapt_BR
dc.contributor.authorCoelho, Camila H.pt_BR
dc.contributor.authorCirelli, Cecíliapt_BR
dc.contributor.authorCrepaldi, Fredericopt_BR
dc.contributor.authorRodrigues-Chagas, Isabela Aurorapt_BR
dc.contributor.authorFurst, Cinthiapt_BR
dc.contributor.authorPimenta, Daniel Carvalhopt_BR
dc.contributor.authorToledo, Juliano Simões dept_BR
dc.contributor.authorFernandes, Ana Paulapt_BR
dc.contributor.authorCosta, Adriana Oliveirapt_BR
dc.date.accessioned2021-02-04T18:28:39Z-
dc.date.available2021-02-04T18:28:39Z-
dc.date.issued2021pt_BR
dc.identifier.citationCarvalho-Silva AC, Coelho CH., Cirelli C, Crepaldi F, Rodrigues-Chagas IA, Furst C, et al. Differential expression of Acanthamoeba castellanii proteins during amoebic keratitis in rats. Exp Parasitol. 2021 Fev;221:108060. doi:10.1016/j.exppara.2020.108060.pt_BR
dc.identifier.urihttps://repositorio.butantan.gov.br/handle/butantan/3511-
dc.description.abstractAmoebic keratitis (AK) is a sight-threatening infection characterized by a severe inflammation of the cornea, caused by the free-living protozoan of the genus Acanthamoeba. Identification of amoebic proteins involved in AK pathogenesis may help to elucidate molecular mechanisms of infection and contribute to indicate diagnostic and therapeutic targets. In this study, we evaluated changes in the expression profile of Acanthamoeba proteins triggered by the invasive process, using an approach involving two-dimensional polyacrylamide gel electrophoresis (2DE PAGE), followed by mass spectrometry identification (ESI-IT-TOF LC-MSn). AK was induced by intrastromal inoculation in Wistar rats, using trophozoites from a T4 genotype, human case-derived A. castellanii strain under prolonged axenic culture. Cultures re-isolated from the lesions after two successive passages in the animals were used as biological triplicate for proteomic experiments. Analysis of the protein profile comparing long-term and re-isolated cultures indicated 62 significant spots, from which 27 proteins could be identified in the Acanthamoeba proteome database. Five of them (Serpin, Carboxypeptidase A1, Hypothetical protein, Calponin domain-containing protein, aldo/keto reductase) were exclusively found in the re-isolated trophozoites. Our analysis also revealed that a concerted modulation of several biochemical pathways is triggered when A. castellanii switches from a free-living style to a parasitic mode, including energetic metabolism, proteolytic activity, control of gene expression, protein degradation and methylation of DNA, which may be also involved in gain of virulence in an animal model of AK.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.format.extent108060pt_BR
dc.languageengpt_BR
dc.relation.ispartofExperimental Parasitologypt_BR
dc.rightsOpen Accesspt_BR
dc.titleDifferential expression of Acanthamoeba castellanii proteins during amoebic keratitis in ratspt_BR
dc.typeArticlept_BR
dc.identifier.doi10.1016/j.exppara.2020.108060pt_BR
dc.identifier.urlhttps://doi.org/10.1016/j.exppara.2020.108060pt_BR
dc.contributor.externalUniversidade Federal de Minas Gerais (UFMG)pt_BR
dc.contributor.externalNational Institute of Allergy and Infectious Diseases (NIAID)pt_BR
dc.contributor.externalUniversidade Federal do Espírito Santo (UFES)pt_BR
dc.identifier.citationvolume221pt_BR
dc.subject.keywordAcanthamoebapt_BR
dc.subject.keywordAmoebic keratitis rat modelpt_BR
dc.subject.keywordProteomicspt_BR
dc.relation.ispartofabbreviatedExp Parasitolpt_BR
dc.identifier.citationabntv. 221, 108060, fev. 2021pt_BR
dc.identifier.citationvancouver2021 Fev;221:108060pt_BR
dc.contributor.butantanPimenta, Daniel Carvalho|:Pesquisador:Docente Permanente PPGTOX|:Laboratório de Bioquímica e Biofísicapt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦APQ-01100-14pt_BR
dc.sponsorship.butantanFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)¦¦red001214pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦406385/2018-1pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦465293/2014-0pt_BR
dc.sponsorship.butantanInstituto NacionalFinanciadora de Estudos e Projetos (FINEP)¦¦01.09.0278.04 e 01.12.0450.03pt_BR
dc.sponsorship.butantanCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)¦¦001pt_BR
dc.sponsorship.butantanConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)¦¦303792 / 2016- 7pt_BR
dc.identifier.bvsccBR78.1pt_BR
dc.identifier.bvsdbIBProdpt_BR
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